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Cloning and Sequence Analysis of the GmPＲ10 Gene Promoter from Soybean( Glycine max L. )(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
568-573
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of the GmPＲ10 Gene Promoter from Soybean( Glycine max L. )
Author(s):
MENG Fan-shan; FAN Su-jie; XU Peng-fei; ZHANG Shu-zhen
( Soybean Ｒesearch Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Ministry of Education，Harbin 150030，China)
Keywords:
Soybean;  GmPＲ10;  Promoter;  Cloning;  GUS staining
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0568
Abstract:
GmPＲ10 was homology with the PＲ10 ( pathogenesis-related proteins 10) gene in other plants. To investigate theexpression and regulation of the GmPＲ10 gene in soybean，the 2 235 bp promoter region of 5’-flanking upstream of GmPＲ10gene was isolated from the genomic DNA of soybean cultivars Suinong 10( with high resistance to Phytophthora sojae in Heilongjiang，China) by PCＲ，defined as pGmPＲ10. It was directionally replaced CaMV35S promoter of the expression vectorpBI121. The plant expression vector of pBI121 /pGmPＲ10 /GUS was used to drive expression of the GUS reporter gene in thepBI121，and the construct was transformed into tobacco leaves by Agrobacterium-mediated transformation. GUS staining resultsshowed that，pGmPＲ10 activity was highly induced by drought ( PEG) ，low temperature ( 4℃) ，salicylic acid ( SA) ，jasmonicacid ( JA) and abscisic acid ( ABA) . Therefore，it was speculated that GmPＲ10 gene may be involved in the process ofplant growth and development with the regulation of plant hormones，as well as the process under abiotic and abiotic stressesresponses to the environment. In addition，sequence analysis by PLACE and PlantCAＲE revealed that pGmPＲ10 containedpromoter general structure TATA-box and CAAT-box，light response element，auxin and cytokinin response element，heatshock element，cold response element，drought responsive element and ABA，SA and JA response elements.

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Last Update: 2016-08-22