|Table of Contents|

Effects of Different Soybean Genotypes on the Transformation Efficiency of Soybeanand Analysis of the T-DNA Insertions in the Soybean Genome(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
562-567
Research Field:
Publishing date:

Info

Title:
Effects of Different Soybean Genotypes on the Transformation Efficiency of Soybeanand Analysis of the T-DNA Insertions in the Soybean Genome
Author(s):
YANG Jing1; XING Guo-jie1; DU Qian2; SUI Li2; GUO Dong-quan1; NIU Lu1; YANG Xiang-dong1
1. Agro-Biotechnology Institute，Jilin Academy of Agricultural Sciences /Jilin Provincial Key Laboratory of Agricultural Biotechnology，Changchun130033，China; 2. Institute of Plant Protection，Jilin Academy of Agricultural Sciences，Gongzhuling 136100，China
Keywords:
Soybean ［Glycine max ( L. ) Merr. ］ genotypes;  Agrobacterium-mediated transformation;  Transformationrate;  T-DNA insertions
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0562
Abstract:
Soybean is usually considered as a recalcitrant leguminous crop to Agrobacterium-mediated transformation. Soybeangenotypes exert significant effects on the transformation efficiency. In this study，two abroad soybean cultivars and 9 domesticcultivated soybeans were employed for Agrobacterium-mediated transformation. PCＲ and LibertyLink strip analysis on the regeneratedplants showed that different soybean genotypes exhibited significant differences in both regeneration and transformationrates. Among them，Jack，Bert，Huachun 6 and Shennong 9 showed higher transformation efficiency than other 7 cultivarswith 6. 45%，3. 80%，3. 24% and 2. 86% of transformation rate. Southern blot analysis was also carried out for the PCＲ-positiveplants derived from Shennong 9. The results showed the integration of T-DNA in the soybean genome with 2. 14% oftransformation rate obtained for the recipient cultivar. Further analysis showed that 75% of the transgenic plants tested containedlow copies( 1-2) T-DNA insertions. Taken together，in this study，four soybean cultivars with high transformation rateswere selected for Agrobacterium-mediated transformation，Huachun 6 and Shennong 9 could be further utilized for developmentof transgenic soybean cultivars.

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Last Update: 2016-08-22