|Table of Contents|

Identification on Insect-resistant Gene of cry1Ab13 Transformation into Glycinemax L. Merr.(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
550-556
Research Field:
Publishing date:

Info

Title:
Identification on Insect-resistant Gene of cry1Ab13 Transformation into Glycinemax L. Merr.
Author(s):
GAO Song1; HE Huan1; WU Nan1; HAN Dan1; QU Jing1; ZHANG Jun1; ZHANG Feng-jing2; WANG Pi-wu1
1. Biotechnology Center of Jilin Agricultural University，Changchun 130118，China;?
2. Changchun Bayi Hospital，Changchun 130061，China
Keywords:
Soybean;  Cry1Ab13;  Plant expression vector;  Insect-resistant
PACS:
S332. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0550
Abstract:
The cry1Ab13 gene used in this experiment is the insect resistant gene，it encodes insecticidal crystal protein andhas certain toxic killing effect on Lepidoptera pests. The plant expression vector of pCambia3300-35S-cry1Ab13 was constructed.The transgenic plants were obtained by pollen-tube pathway transformation of soybean JN28. 15 transformed plants in T1generation were tested by PCＲ，while Southern blotting indicated that only 5 of them showed hybridization bands，and thefunctional fragment was integrated into the soybean genome by single copy. The result of quantitative real-time PCＲ showedthat the cry1Ab13 gene was expressed in soybean leaves and stems，and each plant expression was different， the relatively highexpression was in leaves， the highest was 6. 7，the lowest was 2. 5，while it was 0. 76 and 0. 31 in the stems. The seeds ofPCＲ positive plants in T1 generation and newly hatched larvaes were introduced，and initial insect resistant assay was made bydisk method. Ｒesult suggested that transgenic plant had an obvious resistant effect. Further investigations on the genetic stabilityof cry1Ab13 in transgenic plants and generations are to be carried out.

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Last Update: 2016-08-22