|Table of Contents|

Detection of Bean Pod Mottle Virus by TaqMan-MGB Ｒeal-time IC/TC-ＲT-PCＲ(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年03期
Page:
489-493
Research Field:
Publishing date:

Info

Title:
Detection of Bean Pod Mottle Virus by TaqMan-MGB Ｒeal-time IC/TC-ＲT-PCＲ
Author(s):
CAI Wei1; 2; JIN Jing1; 2; SHEN Jian-guo1; GAO Fang-luan2; LIAO Fu-rong3; WU Zu-jian2
1. Fuqing Entry-Exit Inspection and Quarantine Bureau /Fujian Key Laboratory for Technology Ｒesearch of Inspection and Quaranting，Fuzhou 350300，China;?
2. Institution of Plant Virology，Fujian Agriculture and Forestry University，Fuzhou 350002，China;
3. Xiamen Key Laboratory for Technology Ｒesearchof Inspection and Quarantine，Xiamen 361012，China
Keywords:
TaqMan-MGB real-time IC-ＲT-PCＲ;  TaqMan-MGB real-time TC-ＲT-PCＲ;  Bean pod mottle virus ( BPMV) ;  Detection
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0489
Abstract:
Bean pod mottle virus( BPMV) is regarded as an important quarantine plant pathogen in our country，the risk ofBPMV spread from outside increases under the huge soybean import. TaqMan-MGB real-time IC-ＲT-PCＲ and TaqMan-MGBreal-time TC-ＲT-PCＲ methods were developed to detect BPMV by using soybean seed extracts as materials. Based on the conservedregion of coat protein sequences of BPMV in GenBank，primers and TaqMan-MGB probe were designed. The specificityand sensitivity of these two methods were measured and the comparation of sensitivity of TaqMan-MGB real-time IC-ＲT-PCＲ，TaqMan-MGB real-time TC-ＲT-PCＲ，IC-ＲT-PCＲ and TC-ＲT-PCＲ were operated. Two established methods had a high specificity，the comparison results indicated that TC-ＲT-PCＲ shared 10 － 1 crude extracts，IC-ＲT-PCＲ and TaqMan-MGB real-timeTC-ＲT-PCＲ possessed the same sensitivity reaching 10 － 3 crude extracts while TaqMan-MGB real-time IC-ＲT-PCＲ had thehighest sensitivity about 10 － 5 crude extracts. The sensitivity of TaqMan-MGB real-time IC-ＲT-PCＲ was 102 times more thanTC-ＲT-PCＲ，104 times more than TC-ＲT-PCＲ. It was practical to use the two established methods in sample detection. Sothe established TaqMan-MGB real-time IC/TC-ＲT-PCＲ method could meet the need of detection with specific capture of antibodyor nonspecific capture of tube to catch virion and no need to isdate ＲNA. It provides the basic approach to detect BPMVin imported soybean seeds with required specificity and sensitivity characteristics.

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Last Update: 2016-07-24