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Glutathione Metabolism Different Expression in Ｒesistant and Susceptible SoybeanＲoots after Infected by Heterodera glycines Ichinohe(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年03期
Page:
468-471
Research Field:
Publishing date:

Info

Title:
Glutathione Metabolism Different Expression in Ｒesistant and Susceptible SoybeanＲoots after Infected by Heterodera glycines Ichinohe
Author(s):
SUN Jia-ji; LI Shuang; ZHU Xiao-feng; WANG Yuan-yuan; CHEN Li-jie; LIU Xiao-yu; CHEN Jing-sheng; DUAN Yu-xi
College of Plant Protection，Shenyang Agricultural Uinversity /Plant Protection Station of Liaoning Province，Shenyang 110866，China
Keywords:
Huipizhiheidou;  Glutathione;  Heterodera glycines Ichinohe;  Ｒeal-time Quantitative PCＲ
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0468
Abstract:
The resistant variety Huipizhiheidou and susceptible cultivar Liaodou 15 were tested against soybean cyst nematode( Heterodera glycines，SCN) . The plants were inoculated with eggs of SCN 3 to test the number of the second stage juvenilesand the content of reactive oxygen species. The expression of Glutathion synthesis pathway related genes were detected in theresistant Huipizhiheidou and the susceptible Liaodou 15 by Ｒeal-time Quantitative PCＲ. The results showed that at 5 dpi，nodifference of J2 occurred between Huipizhiheidou and Liaodou 15，at 15 dpi，the number of juveniles invaded roots of HuipizhiHeidouwas less than Liaodou 15. Quantification of reactive species( ＲOS) was higher in Liaodou 15. The relative expressionlevel of glutathione glutamate cysteine ligase was higher in Huipizhiheidou and Liaodou 15 after all inoculated times. Incontrast，the expression of GSHS and hGSHS was significantly lower in Huipizhiheidou than in Liaodou 15 at 15 days. The reductionof glutathione related genes were positively related with resistance of Huipizhiheidou，lead to the oxidation of soybeanroot，which go against to the development of soybean cyst nematode

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Last Update: 2016-07-24