|Table of Contents|

Effect of UV Mutation on Chlorimuron-ethyl Degradation Ability ofAspergillus Niger(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年03期
Page:
455-460
Research Field:
Publishing date:

Info

Title:
Effect of UV Mutation on Chlorimuron-ethyl Degradation Ability ofAspergillus Niger
Author(s):
TAO Bo; PAN Si-yang; WANG Huan; GAO Shi-jie
Agricultural College，Northeast Agricultural University，Harbin 150030，China
Keywords:
UV;  Herbicide Chlorimuron-ethyl;  Aspergillus Niger;  Microbial degradation;  HPLC
PACS:
Q939. 96; S143. 92
DOI:
10.11861/j.issn.1000-9841.2016.03.0455
Abstract:
In this paper，the effect of ultraviolet mutagenesis on chlorimuron-ethyl degradation ability of Aspergillus Niger wassystematic studied. Ultraviolet( UV) method is used to Aspergillus Niger mutagenesis，and a high performance liquid chromatographymethod was established for determination of the degradation rates of stain to chlorimuron-ethyl. The result showedthat，induced mutation by ultraviolet( UV) the total aberration rates of Aspergillus Niger was varied with the UV intensity andirradiation time，greatly influenced by time，the best UV condition was 30 W and 150 s，positive mutation rate was 35. 16%.The degradation rates of herbicide chlorimuron-ethyl by Aspergillus Niger was studied after UV mutagenesis. UV mutagenesiscould significantly improve degradation rates of herbicide chlorimuron-ethyl by Aspergillus Niger，When the UV condition was30 W and 150 s the degradation rate of Aspergillus Niger on chlorimuron-ethyl was the greatest，half-life of chlorimuron-ethylshortened 17. 1 h than before. Finally selected the most significant effect strains H1，its degradation ability increased by73. 16% than the original strains，and continued training after 10 generations degradation of performance and growth rate werestable and could be stable genetic.

References:

［1］ 邹月利，陶波. 除草剂降解真菌对氯嘧磺隆的降解作用［J］.土壤通报，2013，44 ( 6) : 1445-1448. ( Zou Y L，Tao B. Ｒesearchon the effects of different strains on the degradation of herbicidechlorimuron-ethyl［J］. Chinese Journal of Soil Science，2013，44( 6) : 1445-1448. )

［2］ 滕春红，陶波，赵世君. 高效降解真菌对大豆田除草剂氯嘧磺隆的降解特性研究［J］. 大豆科学，2006，25 ( 1) : 58-61.( Teng C H，Tao B，Zhao S J. Study on degradation characteristicsof fungi F8 to degrading herbicide chlorimuron-ethyl［J］. SoybeanScience，2006，25( 1) : 58-61. )
［3］ 汪佳秀，张祥辉，穆文辉，等. 降解菌2N3 对被氯嘧磺隆污染土壤的生物修复［J］. 农药学学报，2010，12 ( 1) : 49-53.( Wang J X，Zhang X H，Mu W H. Bioremediation of chlorimurnethylcontaminated soil by strain 2N3［J］. Chinese Journal of PesticideScience，2010，12( 1) : 49-53. )
［4］ 潘涛，周剑，虞龙. 紫外诱变柠檬酸生产菌黑曲霉的选育［J］. 化学与生物工程，2007( 1) : 50-52. ( Pan T，Zhou J，YuL. Breeding of citric acid producing bacteria Aspergillus Niger byUV mutation［J］. Chemistry and Bioengineering，2007 ( 1) : 50-52. )
［5］ 佘秋生，杨海波，杨冠军，等. 紫外诱变黑曲霉筛选高产果胶酶菌种［J］. 中国酿造，2012，31( 6) : 134-137. ( She Q S，YangH B，Yang G J，et al. Breeding of high pectinase-producing Aspergillusniger strain by UV mutagenesis［J］. China Brewing，2012，31( 6) : 134-137. )
［6］ 程鹏飞，农旭华，余晓玲，等. 红曲霉( Monascus anka As3. 782) 菌种的紫外诱变和产色素的条件优化［J］. 食品研究与开发，2010，31( 9) : 147-152. ( Cheng P F，Nong X H，Yu XL，et al. UV mutagenesis and condition optimization for pigmentproduction from Monascus Anka as 3. 782 strain［J］. Food Ｒesearchand Development，2010，31( 9) : 147-152. )
［7］ 李艾，李云凯，程磊. 紫外诱变酿酒酵母筛选耐高温菌株［J］.唐山学院学报，2013，26( 3) : 35-37. ( Li A，Li Y K，Cheng L.Screening of saccharomyces cerevisiae with temperature toleranceradiated by UV［J］. Journal of Tangshan College，2013，26( 3) :35-37. )
［8］ 王丽宁，赵妍，张宝粉，等. 利用原生质体紫外诱变技术选育耐高温香菇菌株［J］. 微生物学通报，2014，41 ( 7) : 1350-1357. ( Wang L N，Zhao Y，Zhang B F，et al. Breeding thermotolerantstrains of Lentinula edodes by UV induced protoplast mutagenesis［J］. Microbiology China，2014，41( 7) : 1350-1357. )
［9］ 崔培梧，黎继烈，文蓉，等. 紫外线诱变青霉P-M1 选育柚苷酶高产菌株研究［J］. 北方园艺，2009( 2) : 42-44. ( Cui P W，Li J L，Wen Ｒ，et al. Breeding of high-yield naringinase strainfrom Penicillium sp. P-M1 by UV mutation［J］. Northern Horticulture，2009( 2) : 42-44. )
［10］ 牛春华，高岩，李玉秋，等. 紫外诱变选育高产蛋白酶枯草芽孢杆菌［J］. 中国酿造，2011( 12) : 67-69. ( Niu C H，Gao Y，Li Y Q，et al. Selection of high protease-producing strain of Bacillussubtilis by UV mutation［J］. China Brewing，2011 ( 12) : 67-69. )
［11］ 张伟，尚珂，胡又佳，等. 表柔红霉素工程菌的构建及其原生质体紫外诱变［J］. 中国医药工业杂志，2009，40( 1) : 20-23.( Zhang W，Shang K，Hu Y J，et al. Construction of an epidaunorubicinengineering producer and its protoplast mutagenesis by UVirradiation［J］. Chinese Journal of Pharmaceuticals，2009，40( 1) : 20-23. )
［12］ 王永杰，李顺鹏，沈标，等. 有机磷农药降解菌的紫外诱变育种［J］. 应用与环境生物学报，1999，5( 6) : 635-637. ( Wang YJ，Li S P，Shen B，et al. Breeding by ultraviolet ray mutation oforganophosphorous pesticides-degrading strain of bacillus［J］. ChineseJournal of Applied and Environmental Biology，1999，5( 6) :635-637. )
［13］ 王兆守，梁小虾，林凎，等. 拟除虫菊酯类农药降解菌的紫外460 大豆科学3 期线诱变［J］. 华东昆虫学报，2003，12( 2) : 82-86. ( Wang Z S，Li S P，Lin J，et al. Mutation of synthetic pyrethroid insecticidesdegradingstrains by ultraviolet radiation［J］. Entomological Journalof East China，2003，12( 2) : 82-86. )
［14］ 刘辉. 霉菌对磺酰脲除草剂残留解毒效应的研究［D］. 哈尔滨: 东北农业大学，2003. ( Liu H. Effect of Asperilla on the detoxificationof sulfonylurea herbicide［D］. Harbin: Northeast AgriculturalUniversity，2003. )
［15］ 宋艳宇. 氯嘧磺隆在大豆田的残留动态及其降解菌的特性研究［D］. 长春: 吉林农业大学，2006. ( Song Y Y. The residualdynamics in soybean，soiland isolation，identification of chlorimuronethyl-degrading strain［D］. Changchun: Jilin Agricultural University，2006. )
［16］ 丁伟，杨薇，白赫，等. 长残留除草剂氯嘧磺隆降解菌的筛选、鉴定和降解特性［J］. 作物杂志，2007 ( 4) : 88-91. ( DingW，Yang W，Bai H，et al. The screening，identification and degradationcharacteristics of long residual herbicide chlorimuron-ethy［J］. Crops，2007( 4) : 88-91. )
［17］ 滕春红，陶波. 氯嘧磺隆高效降解真菌F8 的分离和鉴定［J］.土壤通报，2008，39( 5) : 1160-1163. ( Teng C H，Tao B. Isolationand identification of a fungi strain F8 capable of degradingchlorimuron-ethyl［J］. Chinese Journal of Soil Science，2008，39( 5) : 1160-1163. )
［18］ 李丽，谢明，周淑云，等. 氯嘧磺隆高效降解细菌的分离与筛选［J］. 中国生物防治，2009，25 ( 1) : 70-73. ( Li L，Xie M，Zhou S Y，et al. Isolation and screening of chlorimuron-ethyl degradingbecteria from soil［J］. Chinese Journal of Biological Control，2009，25( 1) : 70-73. )
［19］ 付峰. 龙须菜紫外诱变育种及抗逆速生优良品系的选育［D］.青岛: 中国海洋大学，2013. ( Fu F. The ultraviolet mutagenesisbreeding and selection of stress tolerance and fast growth strains ofGracilariopsis lemaneiformis［D］. Qingdao: Ocean University ofChina，2013. )
［20］ 于长青，李丽娜. 深黄被孢霉高产花生四烯酸菌株的紫外诱变原生质体育种［J］. 微生物学报，2009，44( 1) : 44-48. ( YuC Q，Li L N. Breeding of arachidonic acid producing Mortierellasabellina by ultraviolet mutation［J］. Acta Microbiologica Sinica，2009，44( 1) : 44-48. )

Memo

Memo:
-
Last Update: 2016-07-24