|Table of Contents|

Screening Strategy of Genetically Modified Soybean Based on QualitativePCＲ Methods(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年03期
Page:
411-417
Research Field:
Publishing date:

Info

Title:
Screening Strategy of Genetically Modified Soybean Based on QualitativePCＲ Methods
Author(s):
LIU Bing; ZHANG Ying; ZHANG Hai-bo; YANG Juan-ni; CHEN Xi; ZHANG Tian
( Shaanxi Seed Administration Bureau / The Crop Seed Quality Testing Center，Xi’an 710018，China)
Keywords:
Genetically modified soybean;  Screening method;  PCＲ method;  Sensitivity
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0411
Abstract:
Genetically modified ( GM) soybean is the largest amount crop of imported genetically modified organism in China.In order to avoid unauthorized genetically modified ( GM) soybean events occurred occasionally，a quick screening method isneeded urgently. According to the inserted gene elements information of commercial domestic and foreign in GM soybean events，a screening strategy was developed for detecting complex molecular structure in GM soybean events based on elementfrequencies． A combination of CaMv35S promoter，CP4-epsps /Bt /Bar /pat genes as screening target elements could screentwelve genetically modified ( GM) soybean events，the event specific primer could detect other three genetically modified( GM) soybean. Ｒesults showed that the detection sensitivity of this method was 1 g·kg － 1. In conclusion，the screening strategyis currently a unique and very valuable tool which could quickly screen out GM soybean detection with high sensitivity andspecificity.

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Last Update: 2016-06-28