|Table of Contents|

Mapping of Gene with Ｒesistance to Soybean Mosaic Virus Strain N1(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年03期
Page:
407-410
Research Field:
Publishing date:

Info

Title:
Mapping of Gene with Ｒesistance to Soybean Mosaic Virus Strain N1
Author(s):
HAN Ying-peng; ZHAO Xue; GAO Sai-nan; LI Hai-yan; LI Wen-bin
Agronomy College，Northeast Agricultural University /Chinese Education Ministry’s Key Laboratory of Soybean Biology /Key Laboratory of NortheasternSoybean Biology and Breeding ( Genetics) of Chinese Agriculture Ministry，Harbin 150030，China
Keywords:
Soybean;  Soybean mosaic virus;  Mapping of resistance gene;  Molecular assistant selection
PACS:
S511
DOI:
10.11861/j.issn.1000-9841.2016.03.0408
Abstract:
Soybean mosaic virus ( SMV) is one of the most widely distributed viral disease in soybean producing areas，whichseriously impact on soybean yield and quality. Molecular marker assisted breeding was one of the most effective methods to selecta resistance line. The aim of this study is to confirm the resistance gene of Dongnong 93-046 through a F8∶9 populationsfrom a cross between Dongnong 93-046 and Pin1246，to map the resistance gene，and to develop associated markers. The resultsshowed resistance line number∶ susceptible line number is 1∶1 in the F8∶9 generation，which showed that the resistance ofDonong 93-046 was controlled by a pair of alleles. A total of 76 SSＲ molecular markers were screened between parents. A totalof 13 SSＲ markers in linkage group F were used to construct a genetic map. The single marker analysis and composite intervalanalysis results show that a Dongnong 93046 resistance gene was located near the SSＲ marker Satt114. A total of 60susceptible lines and 60 resistance lines were used to evaluate accuracy of the marker，the accuracy rate of this marker was80%. It offered a good foundation for molecular marker assisted selection.

References:

［1］ Cho E K，Goodman Ｒ M． Strain of soybean mosaic virus: Classificationbased on virulence in resistant soybean cultivars［J］. Phytopathology，1979，69: 467-470.

［2］ Buss G Ｒ，Ma G，Chen P，et al. Ｒegistration of V94-5152 soybeangermplasm resistance to soybean mosaic virus potyvirus［J］.Crop Science，1997，37: 1987-1988.
［3］ Kiihl Ｒ，Hartwig E． Heritance of reaction to soybean mosaic virusin soybean［J］. Crop Science，1979，19: 372-375.
［4］ 李文福，刘纯燕，高运来，等. 大豆种粒斑驳抗性的遗传分析及基因定位［J］. 作物学报，2008，34( 9) : 1544-1548. ( Li WF，Liu C Y，Gao Y L，et al. Genetic Analysis and mapping of resistancegene to seed coat mottle in soybean［J］. Crop Journal，2008，34( 9) : 1544-1548. )
［5］ Fu S X，Zhan Y，Zhi H J，et al. Mapping of SMV resistance geneＲsc-7 by SSＲ markers in soybean［J］. Genetica，2006，128:63-69.
［6］ Li H C，Zhi H J，Gai J Y. Inheritance and gene mapping of resistanceto soybean mosaic virus strain SC14 in soybean［J］. Journalof Integrative Plant Biology，2006，48( 12) : 1466-1472.
［7］ 郑翠明，常汝镇，邱丽娟. 大豆对SMV3 号株系的抗性遗传分析及抗病基因的ＲAPD 标记研究［J］. 中国农业科学，2001，34( 1) : 1-4. ( Zheng C M，Chang Ｒ Z，Qiu L J. ＲAPD analysis ofresistance gene to soybean mosaic virus［J］. Chinese AgriculturalScience，2001，34( 1) : 1-4. )
［8］ 卢双勇，滕卫丽，韩英鹏，等. 大豆抗花叶病毒及耐疫霉根腐病的SSＲ 分析［J］. 大豆科学，2008，27 ( 5) : 746-750. ( Lu SY，Teng W L，Han Y P，et al. SSＲ analysis of soybean mosaic virusand phytophthora root rot［J］. Soybean Science，2008，27( 5) : 746-750. )
［9］ Lander E S，Green P，Abrahamson J. MapMaker: An interactivecomputer package for constructing primary genetic linkage maps ofexperimental and natural population［J］. Genomics，1987，1:174-181.
［10］ 滕卫丽. 大豆抗花叶病遗传、细胞超微结构分析及基因定位［D］. 哈尔滨: 东北农业大学，2006. ( Teng W L. Inheritance ofresistance to SMV cellular ultastructure analysis and resistancegene mapping in soybean［D］. Harbin: Northeast Agricultural University，2006. )
［11］ Yu Y. GＲFLP and microsatellite mapping of a gene for soybeanmosaic virus resistance［J］. Phytopathology，1994，84 ( 1 ) :60-6479.
［12］ Gore M A，Hayes A J，Jeong S C，et al. Mapping tightly linkedgenes controlling potyvirus infection at the Ｒsv1 and Ｒpv1 region insoybean［J］. Genome，2002，45: 592-599.
［13］ Shi A，Chen P Y，Li X，et al. Genetic confirmation of 2 independentgenes for resistance to soybean mosaic virus in J05 soybeanusing SSＲ markers［J］. Journal of Heredity，2008，99( 6) :598-603

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Last Update: 2016-06-28