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Cloning and Bioinformatics Analysis of GmGLP10 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年03期
Page:
388-393
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatics Analysis of GmGLP10 in Soybean
Author(s):
ZHANG Yu-hangLI Yong-guangWANG Xue-songSUN Ming-yangGOU HanCHEN WeiLI YueLI Wen-bin
Agronomy College of Northeast Agricultural University /Key Laboratory of Soybean Biology in Chinese Ministry of Education /Key Laboratory of SoybeanBiology and Genetics Breeding,Chinese Agriculture Ministry,Harbin 150030,China)ion as an Auxin binding protein to regulate growth and development of soybeanand disease resistance defense responses.
Keywords:
Soybean GmGLP10 gene Cloning Sclerotinia Sclerotiorum Auxin binding protein
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0388
Abstract:
GmGLP10 gene was isolated from soybean Sclerotinia Sclerotiorum resistant cultivar Maple Arrow by PCR method.Bioinformaties analysis showed that GmGLP10 gene encoding a 213 amino acids protein,with a N-glycosylation site and severalphosphorylation sites and was putatively to be a extracellular protein. The Upstream 1 500 bp sequence of GmGLP10 genewere analyzed,multiple cis-elements related to hormone and defense stress responses existed in the GmGLP10 promoter region.Phylogenetic analysis results indicted that GmGLP10 and several Auxin binding proteins( ABPs) had closer genetic relationship,that may have similar functions. The GmGLP10 transcript abundance was significantly increased after Sclerotia infection.These results suggested GmGLP10 may funct

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Last Update: 2016-06-28