|Table of Contents|

Bioinformatics Analysis on Soybean Pathogenesis-related Protein( PＲ-5) and ItsHomology Proteins TPLs(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年03期
Page:
380-387
Research Field:
Publishing date:

Info

Title:
Bioinformatics Analysis on Soybean Pathogenesis-related Protein( PＲ-5) and ItsHomology Proteins TPLs
Author(s):
WEI Lai1; 2; ZHANG Li1; WANG Wei-wei1; YU Zhi-yuan1; YU De-yue2; LIU Li-jun1
1. Soybean Ｒesearch Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，China;?
2. Agronomy College of Nanjing ArgriculturalUniversity，Nanjing 210095，China
Keywords:
Soybean pathogenesis-related proteins;  TLPs;  Evaluation analysis;  Bioinformatics
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0380
Abstract:
Protein PＲ-5 of soybean is one of the PＲs，known as TLPs，which exist in higher plants and can be induced to expressby biotic and abiotic stress( including pathogenic microorganisms，osmotic stress，mechanical damage and phytohormoneetc. ) . In present study，the structure and function of gene Glyma01g42670. 1 and its homologous protein were analyzedthrough bioinformatics，meanwhile 42 soybean TLPs protein sequences were collected to build a phylogenetic tree by softwareMega 4. 1，and all the expression data of TLPs from website SoyBase were analyzed. The results showed: Gene Glyma01g42670.1 coded peptide with 240 amino acids，and the protein was acidic and hydrophobic with pI 6. 26，which had typicaldomain as TLP family. It had N-terminal signal peptide leading protein membrane transportation，but without transmembranehelix structure，and mainly existed in apoplast. It belonged to secreted proteins and inferred to have endoglucanase catalyticactivity. From the results of evaluation analysis，gene Glyma01g42670. 1 was the orthologous gene of gene NP_192902. 1from Arabidopsis，which had the same function and the same origin. From the results of tissue differential analysis，this genemainly expressed in roots and flowers. All the research helps to clarify the function and molecular mechanism of how TLPsgene family did during the plant development.

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Last Update: 2016-06-23