|Table of Contents|

The Detection of Transgenic Soybean GTS 40-3-2 Component in Animal Feed(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年02期
Page:
295-300
Research Field:
Publishing date:

Info

Title:
The Detection of Transgenic Soybean GTS 40-3-2 Component in Animal Feed
Author(s):
YUAN Jian-qin1 ZHAO Jiang-he1 SHI Zong-yong1 LI Jing-yi1 LI Min1 WANG Jun-dong2
1.College of Life Science, Shanxi Agricultural University, Taigu 030801, China;?
2. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China
Keywords:
Animal feed Genetically modified soybean GTS 40-3-2 Qualitative PCR Real-time fluorescence quantitative PCR Detection
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.02.0295
Abstract:
Genetically modified (GM) crops are planted widely to promote the global food production,alleviate the problem to the environment due to the use of pesticides, reduce poverty and hunger, bring ongoing considerable interest for the global environment (especially in the developing countries and regions), economic and social benefits. However, biological security problems from GM crops, as of the date of birth is accompanied by, the debate has never stopped Glyphosate resistant transgenic soybean GTS 40-3-2 is currently approved planting as GM crops in most countries and regions.In addition to extracting soybean oil, soybean meal and other remanining by-products are also used as animal feed. In order to detecting genetically modified ingredients in feed, experiments were carried out on the detection glyphosate resistant soybean of 11 animal feeds around Taigu of Shanxi.Qualitative and quantitative PCR were used to detect GTS 40-3-2, which contians CaMV35Spromoter and NOS terminator, Cp4EPSPS gene and soybean standard Lectin-gene. The detection results showed that 11 of feeds all contained genetically modified resistance to glyphosate GTS 40-3-2 soybean, and the content of each were not identical in 11 of feeds GTS 40-3-2 transformants of the laying hens feed was the lowest. The study provided data support for our GM feed forage safety evaluation system and had important theoretical and realistic significance from the reasonable application of transgenic livestock products by feeding animals producing or GM sources of raw materials products.

References:

[1]Greiner R, Konietzny U. Presence of genetically modified maize and soy in food products sold commercially in Brazil from 2000 to 2005 [J]. Food Control, 2008, 19: 499-505.

[2]Arun , Y.lmaz F, Muratoglu K. PCR detection of genetically modified maize and soy in mildly and highly processed foods [J].Food Control, 2013, 32: 525-531.
[3]James C. Executive summary of global status of commercialized Biotech/GM crops: 2013[Z]. ISAAA Briefs, 2013: 46, Ithaca, New York.
[4]Forte V T, Di Pinto A, Martino C, et al. A general multiplex-PCR assay for the general detection of genetically modified soya and maize [J]. Food Control, 2005,16: 535-539.
[5]GMO Compass Genetically modified food and feed: Authorization in the EU [EB/OL].http://www.gmo.compass.org/eng/gmo/db Accessed 07-05-13, 2015.
[6]Ujhelyi G, Vajda B, Béki E, et al.Surveying the RR soy content of commercially available food products in Hungary [J]. Food Control, 2008, 19: 967-973.
[7]European Parliament and of the Council Regulation (EC) No 1830/2003: Concerning the traceability and labeling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC[J].Journal of Official Statistics, 2003, 268: 24-28.
[8]Miraglia M, Berdal K G, Brera C, et al. Detection and traceability of genetically modified organisms in the food production chain [J]. Food Chem Toxicol, 2004, 42: 1157-1180.
[9]Vijayakumar K R, Martin A, Gowda L R, et al. Detection of genetically modified soya and maize: Impact of heat processing[J].Food Chemical, 2009, 117: 514-521.
[10]金芜军, 贾士荣, 彭于发.不同国家和地区转基因产品标识管理政策的比较[J].农业生物技术学报, 2004, 12(1): 1-7.(Jin W J, Jia S R, Peng Y F. Comparison of labeling policy of genetically modified products in different countries and territories[J].Journal of Agricultural Biotechnology, 2004,12(1):1-7.)
[11]王荣谈, 姜羽, 韦娇君, 等. 转基因生物及其产品的标识与检测[J]. 植物生理学报, 2013, 49(7): 645-654.(Wang R T, Jiang Y, Wei J J, et al.The labeling and detection of genetically modified organisms and their derivates[J]. Plant Physiology Journal, 2013, 49(7): 645-654.)
[12]芦春斌, 张伟, 刘标. 抗草甘膦转基因大豆饲料对雄性小鼠脾淋巴细胞体外增殖的影响[J]. 大豆科学, 2012, 31(2): 291-294.(Lu C B, Zhang W, Liu B. Effects of transgenic soybean feed on proliferation of spleen lymphocyte in male mice[J]. Soybean Science, 2012, 31(2): 291-294.)
[13]农业部1782号令3-2012, 转基因植物及其产品成分检测调控元件CaMV-35S启动子、FMV- 35S启动子、NOS启动子、NOS终止子和CaMV- 35S终止子定性PCR方法[S] (Chinese Standard Agriculture Department public announcement No. 1782-3-2012- 2012. Detection of genetically modified plants and derived products.Qualitative PCR methods for the regulatory elements CaMV 35S promoter, FMV 35S promoter, NOS promoter, NOS terminator and CaMV 35S terminator[S]. Standards Press of China, Beijing, China.
[14]农业部1861号令2-2012, 转基因植物及其产品成分检测耐除草剂大豆GTS 40-3-2及其衍生品种定性PCR方法[S].北京:中国标准出版社,2012(Chinese Standard Agriculture Department public announcement No.1861-2-2012. Detection of genetically modified plants and derived products qualitative PCR method for herbicide-tolerant soybean GTS 40-3-2 and its derivates[S]. Beijing: Standards Press of China, 2012.)
[15]中华人民共和国农业行业标准NY/T675-2003, 转基因植物及其产品检测大豆定性PCR方法[S].北京:中国标准出版社,2012.(Agricultural industry standard of the People′s Republic of China NY/T675-2003 Detection of genetically modified plant organisms and derived products-qualitative PCR methods for soybeans[S].Beijing: Standards Press of China, 2003.)
[16]中华人民共和国国家标准GB/T 19495-5-2004, 转基因产品检测核酸定量PCR检测方法转基因大豆GTS 40-3-2实时荧光PCR检测方法[S].北京:中国标准出版社,2012.(Chinese Standard GB/T 19495-5-2004. Detection of genetically modified organisms and derived products-quantitative PCR methods based on nucleic acid, genetically modified soybeans GTS 40-3-2 real-time PCR detection methods[S]. Beijing:Standards Press of China, 2004.)

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Last Update: 2016-04-04