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Cellular Ultrastructure Analysis in Soybean Resistant Lines and Susceptible Lines of NILs Infected by SMV(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年02期
Page:
280-284
Research Field:
Publishing date:

Info

Title:
Cellular Ultrastructure Analysis in Soybean Resistant Lines and Susceptible Lines of NILs Infected by SMV
Author(s):
MA Na;  LUAN He-xiang;  SHEN Ying-chao;  ZHAO Lin;  LIU Zhi-tao;  ZHANG Kai;  WANG Cheng-kun;  ZHI Hai-jian
National Center for Soybean Improvement of Nanjing Agricultural University/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China
Keywords:
Soybean mosaic virus (SMV); Near isogenic lines (NILs); Ultrastructure
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.02.0280
Abstract:
Cross of Qihuang 1 and Nannong 1138-2 was selected to construct near isogenic lines (NILs) . The ultra-structural alterations of resistant lines and susceptible lines of NILs were studied after 10 and 20 d inoculation with SMV strain SC3. In resistant lines, there were no visible external symptoms and obvious damage of mesophyll cell ultrastructure during the two periods. But in the 20 d inoculation, the virus particles were observed, bound in a region with short form and could not configurate the inclusion bodies. In susceptible lines, lots of thread-like SMV particles, cylindrical inclusion bodies and pinwheel inclusion bodies were observed. Great damages of chloroplasts and mitochondrial emerged in the cell, the chloroplast lamellar structure distorted and swelled, the mitochondrial crest swelled and appeared vague. As the disease developed, the chloroplast and mitochondrial membrane were damaged and degraded in various degree. Shaped growths were found on outer membranes of virus and chloroplast, and increased afterwards. Virus were surrounded by large number of vacuoles and vesicles, which may be relevant to transfer or assembling of the virus.

References:

［1］李振岐，曾士迈. 中国小麦锈病［M］. 北京: 中国农业出版社, 2002. (Li Z Q, Zeng S M. China wheat stripe rust［M］. Beijing:China Agriculture Press, 2002.)

［2］张宏昌. 小麦新抗源一粒葡抗条锈病的组织学和超微结构研究［D］. 杨凌: 西北农林科技大学, 2007. (Zhang H C. Histology and ultrastructure of resistant mechanism of a new wheat material-Yilipu to Puccnia striiformis［D］.Yangling: Northwest Agriculture and Forestry University, 2007.)
［3］康振生. 植物病原真菌的超微结构［M］.中国科学技术出版社, 1995: 5-11. (Kang Z S. The ultrastructure of plant pathogenic fungi［M］. Science in China Press, 1995: 5-11.)
［4］李学湛, Kim K S, Martin E M. 大豆花叶病毒(SMV)感染大豆品种“Lee”叶细胞超微结构的研究［J］. 植物病理学报, 1987, 17(4):235-238. (Li X Z, Kim K S, Martin E M. Ultrastural responses of soybean soyban “Lee” leaf cells to infection with an isolate of soybean mosaic virus［J］. Acta Phytopathologica Sinica,1987, 17(4): 235-238.)
［5］李学湛. 复合病毒感染对大豆叶片细胞超微结构的影响［J］. 电子显微学报, 1991, 10(1): 12-16.（Li X Z. Ultrastructural affect of compound viruses infection to soybean leaf cells［J］. Electron Microscopy Society, 1991, 10(1): 12-16.)
［6］滕卫丽, 李文滨, 韩英鹏, 等. 大豆抗感品种(系)接种SMV1叶片细胞超微结构变化的比较［J］.作物杂志, 2008(1):34-36. (Teng W L, Li W B, Han Y P, et al. Comparison of cellular ultrastructure of different soybean varieties infected SMV1 strain［J］. Crops, 2008(1):34-36.)
［7］滕卫丽, 李文滨, 韩英鹏, 等. SMV3对大豆抗感品种叶片细胞超微结构的影响［J］.作物杂志, 2009(3):21-23.(Teng W L, Li W B, Han Y P, et al. Effects of SMV3 infection on cellular ultrastructure of soybean varieties with different resistance［J］. Crops, 2009(3):21-23.)
［8］智海剑, 盖钧镒, 郭东全, 等. 对大豆花叶病毒不同抗性类型品种的细胞超微结构特征［J］. 南京农业大学学报, 2005, 28(1): 6 - 10.(Zhi H J, Gai J Y, Guo D Q, et al. Ultrastructural characterization of soybeans with different types of resistance to soybean mosaic virus［J］. Journal of Nanjing Agricultural University, 2005, 28(1): 6-10.)
［9］李文龙, 王月明, 侯春燕, 等. 两个不同株系大豆花叶病毒侵染大豆细胞的超微病变比较研究［J］. 河北农业大学学报, 2008, 31(4): 1-6.(Li W L, Wang Y M, Hou C Y, et al. Comparative studies on ultrastructural alteration of soybean infected with two different strains of soybean mosaic virus［J］. Journal of Agricultural University of Hebei, 2008, 31(4): 1-6.)
［10］王晨芳. 小麦与条锈菌互作过程中活性氧迸发的组织学和细胞化学研究［D］. 杨凌:西北农林科技大学, 2008. (Wang C F. Studies on histology and cytochemistry of oxidative burst during wheat Puccinia striiformis f. sp. Tritici interaction［D］.Yangling: Northwest Agriculture and Forestry University doctoral dissertation, 2007.)
［11］Wei T, Huang T S, McNeil J, et al. Sequential recruitment of the endoplasmic reticulum and chloroplasts for plant potyvirus replication［J］. Journal of Virology, 2010, 84:799-809.
［12］Tresnaputra U S, Ehara Y. Electron microscopy of soybean leaf cells infected with five strains of soybean mosaic virus ［J］. Tohoku Joural of Agricultural Research, 1989, 39(2-4): 61-71.

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Last Update: 2016-04-04