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Soybean Responses to High Temperatures Under Drought Stress in the Presence of An Over-expressed GmHSFA1 Gene(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年02期
Page:
257-261
Research Field:
Publishing date:

Info

Title:
Soybean Responses to High Temperatures Under Drought Stress in the Presence of An Over-expressed GmHSFA1 Gene
Author(s):
WEI Lai;  WU Guang-xi;  TANG Xiao-fei;  WANG Wei-wei;  WANG Xing-yu;  LIU Li-jun
Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086， China
Keywords:
GmHSFA1- gene;  Drought and heat;  Soybean
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.02.0257
Abstract:
The research on plant resistance to heat and drought conditions could help to increase plant drought resistance. By using T-7 generation overexpression GmHSFA1 soybean lines as material for this study, changes in plant morphology, gene expression, physiology and biochemistry under two temperature treatments (28℃ and 48℃) were analyzed under drought conditions with soil moistures of 8%-9%.An association analysis was used to screen the plant lines for drought and heat resistance, which laid the foundation for genetic improvement and pyramiding.The results showed that the expression of the GmHSFA1 gene in the overexpression soybean lines clearly increased under drought conditions when the temperature reached 48℃. In T-7-27, the expression increased 22 fold, and the expression of the heat shock protein genes HSP70, HSP22 and HSP17-9 clearly increased 46, 7 and 59-fold, respectively. This research confirmed that the leaf proline content increased and that the malondialdehyde (MDA) content was affected by high temperature and different amplification in different lines. The soluble sugar content clearly increased in response to heat, and T-7-27 exhibited the greatest increase of up to 91%. Heat also clearly changed the photosynthesis rate. Under drought conditions and temperatures of up to 48℃, the transgenic soybean photosynthetic rate decreased, but the decreasing amplitude was less than that of non-transgenic soybean lines.According to a gray-related correlation analysis of multiple indicators, the overexpression GmHSFA1 soybean lines T-7-18 and T-7-27 had the best resistance to drought and heat.

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Last Update: 2016-04-04