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Bioinformatic Analysis of gma-miR1510a and Construction of Its Artificial MiRNA Plant Expression Vectors(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年02期
Page:
239-244
Research Field:
Publishing date:

Info

Title:
Bioinformatic Analysis of gma-miR1510a and Construction of Its Artificial MiRNA Plant Expression Vectors
Author(s):
NI Zhi-yong;  YU Yue-hua;  CHEN Quan-jia;  QU Yan-ying
College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China
Keywords:
Soybean;  gma-miR1510a;  amiRNA;  Promoter
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.02.0239
Abstract:
Abstract:MicroRNAs (miRNAs) are newly discovered extensive endogenous small RNAs, which negatively regulate gene expression at the post-transcription levels.In this study，the target gene and the promoter of gmamiR1510a gene were predicted and analyzed by bioinformatics methodology.Many response elements for abiotic stress, plant hormones, tissue-specific expression and light were predicted by the database search programs PLACE and PlantCARE- PMRD software analysis showed that 9 transcripts were targeted by gma-miR1510a, these targets belonged to disease resistance protein (TIR-NBS-LRR class) and ATP binding.The amiR1510a sequence was engineered into the miRNA and miRNA* of At-MIR319 using overlapping PCR and the amiR1510a backbone was transferred into the plant expression vector pCAMBIA3301.

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Last Update: 2016-04-04