|Table of Contents|

Bioinformatics Analysis of Storage Oil Protein in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年02期
Page:
234-238
Research Field:
Publishing date:

Info

Title:
Bioinformatics Analysis of Storage Oil Protein in Soybean
Author(s):
WANG Wei-jie;  WU Dan;  WANG Jin-peng
College of Life Science, North China University of Science and Technology, Tangshan 063000, China
Keywords:
Soybean; Storage oil; Bioinformatics
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.02.0234
Abstract:
There are closed relationships between the quantity of oil storage and oil synthesis pathway in soybean seed, oil synthesis pathway is complicated process involved in many proteins and enzymes. Storage oil proteins, one of the important proteins, were analyzed by bioinformatics method in the paper.The genome of soybean was download from JGI database, the storage oil gene and related proteins were obtained by database searches and Perl program data mining, 23 genes closely related to oil storage were identified from 1 264 storage oil genes in soybean.By using software such as Protparam, SOPMA, ProtComp and SignalP, the features of protein sequences, physical and chemical properties, secondary structure, subcellular location, and signal peptide were analyzed.Bioinformatics analysis indicated that 23 oil storage genes maps unevenly on 12 soybean chromosomes. Twenty-three oil storage proteins vary in compositions of amino acid (165 and 1 012), isoelectric points (5.90 and 10.03), and exons (5 and 16). Secondary structure prediction results showed that alpha helix and random coil were common structures. Those proteins were located on endoplasmic reticulum, plasmalemma and extracellular.Phylogenetic tree were constructed by neighbor-joining methods basing on the multiple sequences alignments of Clustal W provided by MEGA6 software. Analysis of phylogenetic tree showed that the relationships of 23 storage oil genes and their differences in revolutionary history.

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Last Update: 2016-04-11