|Table of Contents|

Transformation of Heat Shock Protein Gene HSP90 of Rice into Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年02期
Page:
222-227
Research Field:
Publishing date:

Info

Title:
Transformation of Heat Shock Protein Gene HSP90 of Rice into Soybean
Author(s):
YU Zhi-jing1;  SHANG Li-xia1; CAI Qin-an1; MENG Fan-gang2; MA Rui1
1.Agro-Biotechnology Research Institute，Jilin Academy of Agricultural Sciences，Changchun 130033，China；
2.Soybean Research Institute，Jilin Academy of Agricultural Sciences，Changchun 130033，China
Keywords:
Soybean; Drought tolerance; Heat shock protein; Genetic transformation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.02.0222
Abstract:
Soybean is an important food and oil crop in China, and is vital to the national economy.However, the soybean production is limited by drought and salinization.In order to breed the transgenic soybean variety with drought tolerance, the heat shock protein gene .HSP90 from rice was first transformed into soybean mediated with Agrobacterium tumefaciens in this study. The transgenic plants with tolerance to drought can be further selected through over-expression HSP90 gene driven by TMV35S promoter. In this experiment total 4 000 cotyledonary node explants infected and 128 independent transgenic plants were obtained by PCR and Southern blot analysis combining PPT selection. The transformation efficiency was 3.2%. Fifteen transgenic plants with drought tolerance were selected.The transgenic plants obtained in this study would be important for further drought tolerance breeding of soybean.

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Last Update: 2016-04-04