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Cloning and Developmental Stages Expression of the Olfactory Coreceptor Gene in Leguminivora glycinivorella (Lepidoptera: Olethreutidae)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年01期
Page:
124-129
Research Field:
Publishing date:

Info

Title:
Cloning and Developmental Stages Expression of the Olfactory Coreceptor Gene in Leguminivora glycinivorella (Lepidoptera: Olethreutidae)
Author(s):
WANG Yu1; 2;  QIAN Hua1;  JIANG Xin-xin3;  ZHANG Yu-lei1;  SU Ge4;  HAN Lan-lan2;  ZHAO Kui-jun2
1.Agricultural Chemistry Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;?
2.Agronomy College of Northeast Agricultural University, Harbin 150030, China;?
3.Water Conservancy and Civil Engineeing College of Northeast Agricultural University, Harbin 150030, China;?
4.Information Center of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
Keywords:
Leguminivora glycinivorella;  Olfactory receptor;  Gene clone;  Sequence analysis;  Expression quantity of developmental stages
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.01.0124
Abstract:
Olfactory co-receptor is one of the most important proteins in the process of insect identify system.In this study we cloned and analyzed an olfactory coreceptor of L. glycinivorella, which was named LglyOrco.The results showed that the full-length open reading frame of LglyOrco was 1 419 bp, encoding 473 amino acid residues and 3 N.glycosylation sites.The molecular weight of LglyOrco was 53.5 kDa and isoelectric point was 7.12. Blast searching in NCBI showed that the deduced amino acid sequence was highly similar with that of other .Orcos of Lepidoptera with acid sequence identities above 89%.LglyOrco- expression in second and third instar larva were 3 to 4 times higher than the first instar larva, forth and fifth instars larva were only 70% and 50% of the first instar larva respectively. The expression of LglyOrco was 10 times higher in the female adult than in the male adult. This study provides theoretical basis to the researching of L. glycinivorella identify system and control technique of L. glycinivorella.

References:

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Last Update: 2016-02-05