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High Efficient Plant Expression Vector with HSSP Gene and Transformation into Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年01期
Page:
171-175
Research Field:
Publishing date:

Info

Title:
High Efficient Plant Expression Vector with HSSP Gene and Transformation into Soybean
Author(s):
LI Xing-long ; HUO Jian-ling; PANG Tian; BAI Xi
College of Life Science of Northeast Agricultural University，Harbin 150030，China
Keywords:
Soybean;  Sulfur-containing amino acids;  Agrobacterium mediated transformation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.01.0171
Abstract:
Lack of sulfur amino acids limited the nutritional value and commercial value of soybeans.In order to improve the sulfur amino acid content, increase nutritional value of soybeans, this study was designed and synthesized artificially high sulfur amino acid storage protein gene (HSSP) and transformed into soybeans. The plant expression vector pTFGS was constructed with efficient plant expression vector pTF101-1 skeleton,via Agrobacterium-mediated transformation of soybean varieties Dongnong 50, detected 16 transgenic soybeans by PCR and strip, conversion efficiency was 1.94%. Transgenic lines GSDL5 were tissue-specific analysis showed that, HSSP gene overexpressed in seeds, while only trace expression in other tissue sites PCR method using the connector lines GSDL5 genomic sequences flanking the insertion site for cloning, obtained with soybean genome sequence flanking sequences matching 436 bp,HSSP gene is inserted into a non-coding region of chromosome 2.This study got a clear genetic background, and only in soybean seeds overexpressing HSSP transgenic lines GSDL5.

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Last Update: 2016-02-05