|Table of Contents|

Isolation, Identification and Pathogenicity Evaluation of Soybean Bacterial Disease Pathogens(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年01期
Page:
112-117,123
Research Field:
Publishing date:

Info

Title:
Isolation, Identification and Pathogenicity Evaluation of Soybean Bacterial Disease Pathogens
Author(s):
ZHANG Hui1 YU Ren-jing1 ZOU Jia-nan1 XIN Da-wei1 HU Zhen-bang1 ZHANG Yong3 LIU Chun-yan2 CHEN Qing-shan1
1.College of Agricultural Northeast Agricultural University, Harbin 150030, China ;
2. Land Reclamation Research and Breeding Center of Heilongjiang, Harbin 150090, China;
3. Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Keshan 161606,China
Keywords:
Soybean Bacterial pathogen Isolation Identification Pathogenicity
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.01.0112
Abstract:
In order to research soybean bacterial disease in Heilongjiang province, one bacterial pathogen was isolated from Suinong 14 diseased leaves which were collected from Harbin Yanjiagang farm. The pathogen has been carried out antibiotic resistance identification, molecular identification, re-inoculated identification and pathogenicity identification. By Gram staining, pathogen-back test, 16S rDNA molecular identification, the pathogen was Pseudomonas syringae and named Psgneau 2. The pathogen exhibited resistance against two antibiotics, ampicillin and carbenicillin, as three concentration 25,50,100 μg·mL-1, but susceptible to spectinomycin, rifampicin, kanamycin, gentamicin, tetracycline and chloramphenicol. Ten soybean varieties were inoculated with Psgneau 2, of which Suinong 14 was more susceptible than others and Hefeng 35 was the most resistant to Psgneau 2.

References:

[1]丁俊杰.大豆细菌斑点病抗性鉴定的研究概况[J].黑龙江农业科学,2013 (1):132-134.(Ding J J.The research status of the resistance identification to Pseudomonas syringae pv. glycinea[J].Heilongjiang Agicultural Sciences,2013 (1):132-134).

[2]Chiku K,Tsunemi K,Yamamoto M,et al.Defects in D.rhamnosyl residue biosynthetic genes affect lipopolysaccharide structure, motilityand cell-surface hydrophobicity in Pseudomonas syringae pv. glycinea Race 4[J].Bioscience, Biotechnology and Biochemistry,2013,77(3):505-510.
[3]McCann H C,Rikkerink E H A,Bertels F,et al.Genomic analysis of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae provides insight into the origins of an emergent plant disease[J].PLoS Pathogens,2013,9(7):e1003503.
[4]王芳.大豆细菌性病害的识别与综合防治[J].大豆通报,2007(5):9.(Wang F.The identification and integrated prevention of bacterial diseases in soybean[J].Soybean Bulletin,2007(5):9.)
[5]Lee S,Yang D S,Uppalapati S R,et al.Suppression of plant defense responses by extracellular metabolites from Pseudomonas syringae pv. tabaci in Nicotiana benthamiana[J].BMC Plant Biology,2013,13(1):65.[6]Gupta K J, Brotman Y,Segu S,et al.The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco[J].Journal of Experimental Botany,2013,64(2):553-568.
[7]Wrather J A, Koenning S R.Effects of diseases on soybean yields in the United States 1996 to 2007[J].Plant Health Progress,2009.doi:10.1094/PHP-2009-0401-01-RS
[8]Park E W.Effects of bacterial blight on soybean yield[J].Plant Disease,1986,70.
[9]Cross J E,Kennedy B W,Lambert J W,et al.Pathogenic races of the bacterial blight pathogen of soybeans,Pseudomonas glycinea[J].Plant Disease Reporter, 1966,50(8):557-560.
[10]Prom L K,Venette J R.Races of Pseudomonas syringae pv. glycinea on commercial soybean in eastern North Dakota[J].Plant Disease,1997, 81(5):541-544.
[11]张佳环,高洁,许庆国,等.大豆品种对大豆细菌性斑点病的抗性鉴定[J].大豆科学,2000,19(2):180-183.(Zhang J H, Gao J, Xu Q G, et al.Identification of the resistance of soybean varieties to bacterial blight disease of soybean[J].Soybean Science,2000,19(2):180-183.)
[12]Mukherjee D, Lambert J W,Cooper R L,et al.Inheritance of resistance to bacterial blight (Pseudomonas glycinea Coerper) in soybeans (Glycine max L)[J].Crop Science,1966,6(4):324-326.
[13]Wrather J A,Kendig S R,Anand S C,et al.Effects of tillage, cultivar, and planting date on percentage of soybean leaves with symptoms of sudden death syndrome[J].Plant Disease,1995,79(6):560-562.
[14]Staskawicz B,Dahlbeck D,Keen N,et al.Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea[J].Journal of Bacteriology,1987,169(12):5789-5794.
[15]张淑珍,徐鹏飞,吴俊江,等.黑龙江省大豆品种对细菌性斑点病的田间抗病性调查及室内接种鉴定分析[J].东北农业大学学报,2006,37(5):588-591.(Zhang S Z,Xu P F,Wu J J,et al.Study on the identification of the resistance of soybean varieties(lines) to Pseudomonas syringae pv. Glycinea in field and inoculation indoors[J].Journal of Northeast Agricultural University,2006,37(5):588-591.)
[16]孙永吉,刘宗麟,刘玉芝,等.大豆品种资源抗细菌斑点病鉴定与评价[J].大豆科学,1989,8(2):185-189.(Sun Y J,Liu Z L,Liu Y Z,et al.Evaluation and identification of soybean cultivars and lines for resistance to bacterial blight[J].Soybean Science,1989,8(2):185-189.)

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Last Update: 2016-02-05