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Effect of Soil Disturbance to the Biological Control of Fermentation Filtrate of Parasitic Fungi on Soybean Cyst Nematode in Glasshouse(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年01期
Page:
106-111
Research Field:
Publishing date:

Info

Title:
Effect of Soil Disturbance to the Biological Control of Fermentation Filtrate of Parasitic Fungi on Soybean Cyst Nematode in Glasshouse
Author(s):
LU Jian-cong1; 2;  XU Yan-li2;  SONG Jie1; 2
1.Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences/Key Laboratory of Mollisols Agroecology, Harbin 150081, China;?
2.College of Agriculture, Northeast Agricultural University, Harbin 150040, China
Keywords:
Disturbance;  Soybean cyst nematode;  Parasitic fungi;  Fermentation filtrate
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.01.0106
Abstract:
Soybean cyst nematode (SCN), Heterodera glycines, remains a major yield-limiting pathogen of soybean. Natural suppression of SCN exists and becomes increasingly attractive. However, ecological mechanism leading to the suppressive state of cyst nematode is rarely studied.The glasshouse experiments were performed to determine the effects of soil disturbance and fermentation filtrate of parasitic fungal on soybean cyst nematode.Soil disturbance was simulated by soil passing through a sieve(aperture 0.5 cm) and compared with no-disturbance (non-sieve) treatment. Different fermentation filtrate of parasitic fungi were mixed by applying.Paecilomyces lilacinus, Fusarium spp. and Pochonia chlamydosporium.Density of SCN were investigated 35 days after soybeans planted in each pot- It showed that the SCN female, density of cyst, egg and J2 were significantly reduced with 20.0%-35.9%, 18.3%-35.0%, 21.5%-38.5% and 14.6%-35.3% respectively in the treatment of soil disturbance with mixed fermentation filtrate compared with soil disturbance without mixed fermentation, and the disturbance of the 4th mixed fermentation filtrate on soybean cyst nematode density was the most significantly. Without soil disturbance, applying mixed fermentation filtrate could reduce the SCN female, density of cyst, egg and J2 by 19.5%-32.7%, 19.5%-35.2%, 21.1%-37.8% and 8.0%-27.6% respectively.Compared with control treatments, there were no significant difference in the population of SCN female, when J2 treated with different mixed fermentation filtrate. This study also showed that soil disturbance could stimulate the growth of soybean, and increase the fresh weight of soybean plants, but there were no significant difference between those without disturbance.These results indicated that mixed fermentation filtrate could inhibit the development of the SCN female, density of cyst, egg and J2.Soil disturbance could promote preventive effectiveness of fungus metabolism in glasshouse and play an active role on reducing the population density of SCN. Fungi inhibit the growth and development of SCN, coordination application of soil disturbance interference and fungal metabolites might induce the population density of SCN and affect its biological control efficiency in soil.

References:

［1］刘维志. 植物病原线虫学 ［M］.北京: 中国农业出版社, 2000: 28l-294. (Liu W Z. Plant pathogenic nematodes ［M］. Beijing: China Agriculture Press, 2000: 28l-294.)

［2］Hartwig E E. Breeding productive soybean cultivars resistant to the soybean cyst nematode for the southern United States［J］. Plant Disease, 1981, 65(4): 303-305.
［3］许艳丽. 土壤环境对大豆胞囊线虫卵孵化影响及线虫分子诊断研究 ［D］. 哈尔滨: 东北农业大学, 2004.(Xu Y L.The effects of soil environment on soybean cyst nematode egg hatch and nematode molecular diagnosis ［D］.Harbin：Northeast Agricultural University, 2004.)
［4］Chen S Y. Suppression of Heterodera glycines in soils from fields with long-term soybean monoculture ［J］. Biocontrol Science and Technology, 2007, 17(2): 125-134
［5］孙玉秋, 许艳丽, 李春杰, 等.作物轮作系统对土壤中大豆胞囊线虫胞囊量和单胞囊卵量的影响 ［J］.农业系统科学与综合研究, 2011, 27(2): 298-251. (Sun Y Q, Xu Y L, Li C J, et al. Influence of cropping rotation systems on the volume of cysts and eggs in a single cyst of soybean cyst nematode ［J］. System Sciences and Comprehensive Studies Agriculture, 2011, 27(2): 298-251.)
［6］靳学慧, 辛惠普, 郑雯, 等. 长期轮作和连作对土壤中大豆胞囊线虫数量的影响 ［J］. 中国油料作报, 2006, 28(2): 189-193. (Jin X H, Xin H P, Zheng W, et al. The influence of soil on the long term rotation and continuous collision on soybean cyst nematode ［J］.Chinese Journal of Oil Crop Sciences, 2006, 28(2): 189-193.)
［7］陈立杰, 段玉玺, 范圣长, 等. 大豆胞囊线虫病的生防因子研究进展 ［J］. 西北农林科技大学学报(自然科学版), 2005, 33(增):190-194.(Chen L J, Duan Y X, Fan S C, et al.Advances in antagonists of soybean cyst nematode ［J］. Journal of Northwest A ＆ F University (Natural Science Edition), 2005, 33(S): 190-194.)
［8］卢明科, 潘沧桑, 李舟. 厚垣轮枝孢菌(Verticillium chlamydosporium)防治植物线虫研究进展 ［J］. 西北农林科技大学学报（自然科学版）, 2004, 32(4): 103-105.(Lu M K, Pan C S, Li Z.Advances of studies of Verticillium chlamydosporium as a biological control agent for plant parasitic nematodes ［J］.Journal of Northwest A & F University (Natural Science Edition), 2004, 32(4): 103-105.)
［9］赵晓晖, 许艳丽. 镰刀菌发酵液对大豆胞囊线虫的抑制作用 ［J］.大豆科学, 2011, 30(3): 468-470. (Zhao X H, Xu Y L. Inhibition of Fusarium.spp fermented filtrates on soybean cyst nematode ［J］ Soybean Science, 2011, 30(3): 468-470.)
［10］Sun M H, Gao L, Shi Y X, et al.Fungi and actinomycetes associated with Meloidogyne spp. eggs and females in china and their biocontrol potential ［J］.Journal of Invertebrate Pathology, 2006, 93: 22-28.
［11］孙玉秋, 许艳丽, 李春杰, 等. 东北地区大豆胞囊线虫卵定殖真菌的多样性研究 ［J］.华北农学报, 2011, 26(2)：233-238. (Sun Y Q, Xu Y L, Li C J, et al. Mycofloras in eggs of soybean cyst nematode in northeast China ［J］. Acta Agriculture Boreall-Sinica, 2011, 26(2)：233-238.)
［12］陈立杰, 刘小杰, 段玉玺, 等. 轮作和连作田大豆胞囊线虫胞囊上真菌定殖动态 ［J］ 大豆科学, 2009, 28(2)：266-270. (Chen L J, Liu X J, Duan Y X, et al. Dynamics of fungial colonization on cyst in rotation and continuous cropping of soybean ［J］.Soybean Science, 2009, 28(2)：266-270.)
［13］赵晓晖, 许艳丽. 厚垣轮枝菌发酵液对大豆胞囊线虫的抑制作用 ［J］. 大豆科技，2011(2):19-25. (Zhao X H, Xu Y L. Inhibition of Verticillium chlamydosporium fermented filtrates on soybean cyst nematode ［J］.Soybean Science and Technology, 2011(2)：19-25.)
［14］赵晓辉. 大豆胞囊线虫抑制性土壤中寄生真菌及其作用研究 ［D］.哈尔滨：东北林业大学, 2011.(Zhao X H.Suppressive soil of soybean cyst nematode and parasitic fungi ［D］. Harbin: Northeast Forestry University, 2011.)
［15］葛红莲, 郭坚华, 祁红英, 等. 复合菌剂AR99防治辣椒青枯病 ［J］. 植物病理学报, 2004, 34(2)：162-165.(Ge H L, Guo J H, Qi H Y, et al. Biological control of Capsieum baeterial wilt by compound baeterial mixture AR99 ［J］. ACTA Phytopathologica Sinica, 2004, 34(2)：162-165.)
［16］王波, 李红梅, 王碧, 等. 淡紫拟青霉与放线菌代谢物复配对南方根结线虫的防治 ［J］. 南京农业大学学报, 2009, 32(1)：55-60.(Wang B, Li H M, Wang B, et al. Biological control of Meloidogyne incognita by combination of Paecilomyces lilacinus and Actinomycetes spp. ［J］. Journal of Nanjing Agricultural University, 2009, 32(1)：55-60.)
［17］钱洪利. 明尼苏达被毛袍对大豆胞囊线虫作用的研究 ［D］. 哈尔滨：东北农业大学, 2009.(Qian H L.The effect of Hirsutella minnestensisisa on soybean cyst nematode ［D］.Harbin: Northeast Agricultural University, 2009.)
［18］Bao Y, Neher D A, Chen S. Effect of soil disturbance and biocides on nematode communities and extracellular enzyme activity in soybean cyst nematode suppressive soil ［J］. Nematology, 2011, 13(6)：687-699.
［19］Jenkins W R. A rapid centrifugal flotation technique for separating nematodes from soil ［J］. Plant Disease, Report, 1964, 48：692
［20］范圣长, 段玉玺, 陈立杰.大豆胞囊线虫胞囊内寄生真菌研究 ［J］.大豆科学, 2004, 23(1)：71-74. (Fan S C, Duan Y X, Chen L J. The reserach on the cyst entoparasitic fungi of soybean cyst nematode ［J］.Soybean Science, 2004, 23(1)：71-74.)

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Last Update: 2016-02-05