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Construction of EMS Mutant Library and the Evaluation of Important Agronomic Traits of M1?in Glycine max(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年01期
Page:
64-69
Research Field:
Publishing date:

Info

Title:
Construction of EMS Mutant Library and the Evaluation of Important Agronomic Traits of M1?in Glycine max
Author(s):
MENG Yong-jie;  CHEN Feng;  SHUAI Hai-wei;  LIU Wei-guo;  DU Jun-bo;  LIU Jiang;  SHU Kai;  YANG Wen-yu
Agronomy College of Sichuan Agricultural University/Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of China, Chengdu 611130, China
Keywords:
Keywords: Soybean;  EMS;  Mutant pool;  Agronomic traits
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.01.0064
Abstract:
Sufficient mutant pool is one of the most important preconditions for molecular biology and plant genetics studies.Lacking of enough mutants of soybean is a serious constraint to soybean functional genomics researching.In this study, soybean mutant pool was constructed by treating seeds of Nandou 12, which is prevailed in Southwestern China, by Ethyl Methyl Sulfone (EMS) treatment.The results showed that, emergence rate of M1?seeds in field barely reached to 34.85%, which indicated that EMS significantly inhibit the emergence of soybean seeds. Analysis of the important agronomic traits, including plant height, pods per plant and so on, of 913 plants of M1?generation showed that, plant height, pods per plant and internodes number were significantly different with Nandou 12.Compared to Nandou 12, 18.07% of the pool showed the dwarf phenotype; 11.06% revealed more pods per plant, and 3 sterile mutants were observed.Furthermore, the range (R) of internodes number of M1?lines reached to 18.Taken together, these results demonstrated that soybean mutant pool had been constructed primarily. Classification of single mutant and cloning of the corresponded gene(s) would be focused on the following studies, which would has a profound effect on soybean functional genetics study and screening perfect soybean varieties for maize-soybean stripe intercropping system.

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Last Update: 2016-01-28