|Table of Contents|

Bioinformatic Analysis of gma-miR1514b and Construction of Its Artificial miRNA Plant Expression Vectors(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年01期
Page:
46-51
Research Field:
Publishing date:

Info

Title:
Bioinformatic Analysis of gma-miR1514b and Construction of Its Artificial miRNA Plant Expression Vectors
Author(s):
NI Zhi-yong;  YU Yue-hua;  CHEN Quan-jia;  QU Yan-ying
College of Agronomy, Xinjiang Agricultural University / Key Laboratory of Agricultural Biological Technology, Xinjiang Agricultural University, Urumqi 830052, China
Keywords:
Soybean;  gma-miR1514b;  amiRNA;  Promoter
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.01.0046
Abstract:
MicroRNAs play fundamental regulatory roles in various aspects of plant development and adaptive responses to stresses. In this study，the target gene and the promoter of gma-miR1514b gene were predicted and analyzed by bioinformatics methodology PMRD database analysis showed that precursor sequence length of gma-miR1514b was 109 bp, located in soybean 1 497 668-1 497 776［+］of chromosome 17, and its mature sequence length was 21 bp. The predicted transcription start site of gma-miR1514b was found within 762 bp upstream of the precursor sequence.The database of PLACE and PlantCARE analysis showed that the promoter sequence contains basic elements TATA-box, CAAT-box and some cis-acting elements involved in response to light, stresses and plant hormones PMRD software analysis showed that 5 transcripts were targeted by gma-miR1514b, these targets were homologous with CA2, NTL9 and ANAC014 genes in Arabidopsis, respectively. The amiR1514b sequence was engineered into the miRNA and miRNA* of At-MIR319 using overlapping PCR and the amiR1514b backbone was transferred into the plant expression vector pCAMBIA3301.

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Last Update: 2016-01-28