|Table of Contents|

Cloning and Bioinformatics Analysis of MPBQ-MT Gene in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年01期
Page:
39-45
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatics Analysis of MPBQ-MT Gene in Soybean
Author(s):
NIE Teng-kun; ZHAO Lin; LI Hai-yan; LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Breeding Genetics of Chinese Agriculture Ministry，Northeast Agricultural University，Harbin 150030，China
Keywords:
Soybean; Vitamin E; MPBQ-MT gene; Bioinformatics analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.01.0039
Abstract:
Cloned the 2-methyl-6-phytyl-1,4-benzoquinone methyltransferase (MPBQMTase) of soybean and obtained the cDNA encoding MPBQMTase using Hefeng 25 and Bayfield as materials. The cDNA sequence analysis showed that the cDNA was 1 029 bp in length.Ten latent SNPs were found in CDS of MPBQ-MT gene in soybean by sequences blast.The amino acid sequence and protein structure of MPBQ-MT gene were analyzed by bioinformatics method. The result showed that the CDS region of MPBQ-MT gene encoded 342 amino acid residues with the most content of Valine which accounted for 8.8% of the total amino acids and the least content of Cysteine which accounted for 1.2% of the total amino acids N-terminal of MPBQ-MT protein contains chloroplast transit peptide composed by 58 terminal amino acids and MPBQ-MT was hydrophilic protein. The secondary structure of MPBQ-MT protein was different in Hefeng 25 and Bayfield.About 25.44% and 27.49% of the protein sequence were alpha helixes respectively in the two varieties, about 9.36% and 9.06% of the protein sequence were beta turn, 40.35% and 38.60% of the protein sequence were random coil 24.85% of the protein sequence were lamellar structure in both varieties. S-adenosyl methionine binding sites exist in MPBQ-MT protein of both varieties.The three stage structure analysis showed 11 continuous alpha helixes and 8 continuous film layer structure exist in MPBQ-MT protein. The model of MPBQ-MT was obtained based on the three stage structure of MPBQ-MT protein and biological function analysis of MPBQ-MT. Phylogenetic tree indicated the relationship of MPBQ-MT protein was close in soybean and bean.

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Last Update: 2016-01-28