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Cloning of the Soybean △1-Pyrroline-5-Carboxylate Genes GmP5CS1 and GmP5CS2 and Construction of Its Overexpression Vectors(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年01期
Page:
25-30,38
Research Field:
Publishing date:

Info

Title:
Cloning of the Soybean △1-Pyrroline-5-Carboxylate Genes GmP5CS1 and GmP5CS2 and Construction of Its Overexpression Vectors
Author(s):
GAO Ming-xiao;  GUO Na;  XUE Chen-chen;  WANG Hai-tang;  ZHAO Jin-ming;  XING Han
Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China
Keywords:
Glycine max L Merr;  GmP5CS gene;  Gateway technology
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.01.0025
Abstract:
In this study, we cloned two genes encoding soybean △ 1-?pyrroline-5- carboxylic acid synthase(P5CS) by homology cloning method of cultivation from the strong drought tolerant soybean (Glycine max) variety Qihuang 22, named GmP5CS1 and GmP5CS2. The amino acid sequence analysis found that GmP5CS1 contained a length of 2 163 bp ORF, encoding 720 amino acids, isoelectric point (pI) was 6.70, protein was weak acid, molecular weight was 78.38 kDa; ?GmP5CS2 contained a length of 2 271 bp ORF, encoding 756 amino acids, isoelectric point(pI) was 6.10, acidic protein, molecular weight was 82.18 kDa. Phylogenetic analysis showed that GmP5CS1 affinity with Medicago sativa highest, GmP5CS2 affinity with Vigna unguiculata highest The organization expression analysis found that GmP5CS1 and GmP5CS2 genes expressed in all soybean organizations.Gene relative expression in leaf and root is higher, relative expression in the stem is middle, the relative expression of flowers and seed is relatively lower A plant over-expression vector of pEarleyGate103-GmP5CS was constructed by Gateway technology, and was transferred into Agrobacterium EHA105, which would lay a foundation of its conversion into the plant and provide a foundation for further studying the function of GmP5CS gene in soybean

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Last Update: 2016-01-28