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Construction of Yeast Hybrid cDNA Library and Screening of Interaction Proteins of GmSPX3 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年01期
Page:
18-24
Research Field:
Publishing date:

Info

Title:
Construction of Yeast Hybrid cDNA Library and Screening of Interaction Proteins of GmSPX3 in Soybean
Author(s):
ZHOU Xi;  ZHANG Jing-yao;  YAO Min-lei;  XIE Feng-bin;  GAI Jun-yi;  YANG Shou-ping
Soybean Research Institute/National Center for Soybean Improvement/MOA Key Laboratory for Biology and Genetic Improvement of Soybean(General)/National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
Keywords:
Soybean［Glycine max (L) Merr］;  GmSPX3 gene;  Gateway technology;  Yeast hybrid cDNA library;  Yeast two-hybrid
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.01.0018
Abstract:
To study the mechanism of GmSPX3 in response to low phosphorus stress and search for the interaction proteins of GmSPX3 in soybean, the present research constructed yeast hybrid cDNA library from the soybean root treated with low phosphorus by gateway technology Furthermore, yeast hybrid cDNA library was screened through the method of yeast two-hybrid using pGBKT7-GmSPX3 fusion protein as bait. After color reaction of validation, 21 positive clones were obtained. Two transcription factors GmUNE12 and GmPosF21 were chosen as the candidate genes for cloning by bioinformatics analysis. Two AD vectors of pGADT7-GmUNE12 and pGADT7-GmPosF21 were constructed. The results of one to one interaction demonstrated that GmUNE12 and GmPosF21 proteins interacted with GmSPX3 bait protein, respectively

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Last Update: 2016-01-28