|Table of Contents|

Validation of Selected gma-miR1508a Targets and Construction of Its Plant Expression Vectors(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年06期
Page:
1090-1092
Research Field:
Publishing date:

Info

Title:
Validation of Selected gma-miR1508a Targets and Construction of Its Plant Expression Vectors
Author(s):
NI Zhi-yong;  YU Yue-hua;  REN Yan-ping;  CHEN Quan-jia;  QU Yan-ying
College of Agronomy, Xinjiang Agricultural University/ Key Laboratory of Agricultural Biological Technology, Xinjiang Agricultural University, Urumqi 830052, China
Keywords:
Soybean;  gma-miR1508a;  Target gene;  Plant expression vector
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.06.1090
Abstract:
MicroRNAs (miRNAs) are endogenous, noncoding, small RNAs that have essential regulatory functions in plant growth, development, and stress response processes.In this study, one target gene of gma-miR1508a, Glyma16g27800, was verified using a modified 5′ RLM-RACE (RNA ligasemediated rapid amplification of 5′ cDNA ends) assay. To generate an overexpression construct that constitutively overexpressed gma-miR1508a under the control of a cauliflower mosaic virus 35S promoter, a 101 bp fragment flanking the miRNA sequence including the fold-back structure was amplified from soybean genomic DNA by PCR. The amplified fragment was subcloned into the pCAMBIA3301 vector. PCR and sequencing results suggest that plant expression vector of gma-miR1508a gene was constructed successfully.This constructed vectors provided an effective too1 for the further study of gma-miR1508a gene function.

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Last Update: 2016-01-07