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Study on the Mechanism of Clump Bud Generation Induced by Polyamines in Soybean Cotyledon Node Callus(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年06期
Page:
1006-1010
Research Field:
Publishing date:

Info

Title:
Study on the Mechanism of Clump Bud Generation Induced by Polyamines in Soybean Cotyledon Node Callus
Author(s):
GAO Feng-ming HU Wen-ting SU Guo-xing
School of Basic and Biological Science, Soochow University, Suzhou 215123, China
Keywords:
Soybean cotyledon node Polyamines oxidative degradation Hydrogen peroxide Clump buds
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.06.1006
Abstract:
Cotyledon node callus of soybean seedlings was used to study the role of hydrogen peroxide from polyamine degradation in induced generation of clump buds in soybean cotyledon node callus.The results showed that three of the exogenous polyamines significantly enhanced clump buds formation of soybean cotyledon node callus, 0.5 mmol?L-1Put was more effective than Spm, but less than Spd. Meanwhile cyclohexylamine (CHA), an inhibitor of Spd synthase, displayed a strong inhibitory effect on the formation of clump buds. Accordingly, activity of diamine oxidase and levels of hydrogen peroxide in the explants of cotyledon node were greatly increased. Interestingly, polyamine-increased differentiation of clump buds was strongly reduced after the application of diamine oxidase inhibitor-aminoguanidine (AG) and H2O2?scavenger ?dimethyl thiourea (DMTU), respectively.Probably, the effects of AG and DMTU on clump buds differentiation were associated with decreases in CuAO activity and the level of H2O2?caused by AG treatments, and H2O2scavenging by DMTU. These results suggest that hydrogen peroxide from polyamine degradation is potentially involved in inductive formation of clump buds in soybean cotyledon node callus.

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Last Update: 2016-01-06