|Table of Contents|

Exploiting SSR Loci Related with Soybean Oil and Protein Content by Using Association Analysis in Natural Population(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年06期
Page:
977-981
Research Field:
Publishing date:

Info

Title:
Exploiting SSR Loci Related with Soybean Oil and Protein Content by Using Association Analysis in Natural Population
Author(s):
YU Zhi-yuan1; WANG Wei-wei1; WEI Lai1; CHEN Qing-shan2; ZHAO Gui-xing1; QI Zhao-ming2; LI Jie2; LIU Li-jun1
1.Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China；
2.Soybean Research Institute of Northeast Agricultural University, Harbin 150030, China
Keywords:
Soybean;  Oil content;  Protein content;  Association analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.06.0977
Abstract:
In order to study the oil and protein content in resource population of soybean, and to select trait related markers, 327 soybean breeding-used cultivars from Northeast China were used as experimental materials. 186 pairs of SSR (simple sequence repeat) markers, distributed on 20 chromosomes of soybean, were selected to detect genotypes of all testing materials, Software STRCTURE 2.3.4 was used to analysis population structure and experimental material were divided into 7 subgroups.The MLM (Mixed Linear Model) process of software TASSEL 3.0 was used to associate oil and protein contents trait data with SSR marker data.33 associated SSR loci were found highly significant (P＜0.01) related with contribution rate greater than 1%. Among them, 12 loci were significantly associated with oil content, explaining 2.7%-10.08% of variation, 11 loci were significantly associated with protein content, explaining 2.4%-9.08% variation.There are 6 identical loci associated with both oil and protein content, which are Satt005, Satt117, Satt565, Satt469, Satt594 and Satt546. Explanation rate of Satt594 is the highest, which is 10.05% for oil content variation and 9.08% for protein content variation.This study provides information for molecular assisted breeding of soybean with high oil and protein content.

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Last Update: 2016-01-05