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Isolation and Sequence Analysis of Transcription Factor GmWRKY53 from Soybean (Glycine max)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年06期
Page:
972-976,981
Research Field:
Publishing date:

Info

Title:
Isolation and Sequence Analysis of Transcription Factor GmWRKY53 from Soybean (Glycine max)
Author(s):
BI Ying-dong1; 2;  LI Wei2;  WANG Ping3;  LIU Ming2;  LI Wan2;  LIU Miao2;  XIAO Jia-lei2;  LAI Yong-cai2
1.Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme/Northeast Forestry University Postdoctoral Programme,Harbin 150086,China;?
2.Crops Tillage and Cultivation Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;?
3.Information Center of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
Keywords:
Transcription factor; WRKY;  W-box; Soybean
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.06.0972
Abstract:
The WRKY protein is a super family of transcription factors unique to plants which was so named as all family members contain a conserved amino acid sequence WRKYGQK and special zinc finger motif at its N-terminal end. WRKY protein may participate in regulating the expression of a wide range of genes involved in various biological processes such as development, metabolism and response to a range of abiotic and biotic stresses. We characterized a predicated transcription factor GmWRKY53 from soybean using the yeast one hybrid system with the bait vector which had W-box of the AtNPR1(Arabidopsis thaliana) promoter related with pathogen defense.It contained one conserved domain, and showed similarity with AtWRKY family members in both overall amino-acid sequences and the secondary structure arrangement within the DNA-binding motifs. In yeast one-hybrid system, GmWRKY53 can specially activate the genes fused with the promoter containing W-box of the AtNPR1 promoter. The study on transcription factor WRKY of soybean is conduced to understand the regulation of various physiological programs of soybean, including pathogen defense, senescence and development.

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Last Update: 2016-01-05