|Table of Contents|

Association of GmDGK7 and GmTPR Genes with Oil Related Traits in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年06期
Page:
938-944
Research Field:
Publishing date:

Info

Title:
Association of GmDGK7 and GmTPR Genes with Oil Related Traits in Soybean
Author(s):
LU Liang;  HE Jian-bo;  MIAO Long;  WANG Xiao-ting;  LIU Fang-dong;  WANG Xin-tong;  GAI Jun-yi;  LI Yan
National Center for Soybean Improvement /National Key Laboratory of Crop Genetics and Germplasm Enhancement / MOA Key Laboratory for Biology and Genetic Improvement of Soybean, Nanjing Agricultural University, Nanjing 210095, China
Keywords:
Soybean;  Fatty acids;  Single nucleotide polymorphism;  Functional molecular markers
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.06.0938
Abstract:
Soybean is the most important oil crop in the world, which oil production accounts for more than a quarter of global vegetable oil production, thus increasing the oil content and oil composition in soybean seed is essential for soybean breeding.However, there are limited molecular markers derived from functional genes in soybean breeding program.Two soybean genes that are homologous to known proteins involved in oil metabolic pathways were chosen to develop molecular markers in this study, including genes encoding a diacylglycerol kinase 7 (DGK7) and a pentatricopeptide repeat-containing protein (TPR), respectively. Based on the position and type of mutation of the single nucleotide polymorphisms (SNPs) within these two genes, we selected SNP1 located in the 5′-untranslated region (5′-UTR) of GmDGK7 and SNP2 located in the GmTPR exon to design dCAPS markers.Association mapping of SNP1 and SNP2 with the oil related traits among 200 soybean released cultivars showed that SNP1 in GmDGK7 is significantly (P = 6.21×10-5) associated with oil content, SNP2 in GmTPR is significantly associated with the content of oil, stearic acid, oleic acid and linoleic acid (P values were 4.9×10-11, 1.6×10-6, 3.4×10-5?and 4.09×10-8, respectively).Fourteen soybean cultivars with high oil content (＞ 23%) and 5 cultivars with high oleic acid (＞ 33%) were identified in this mapping population.These cultivars and dCAPS markers could be used in marker-assisted breeding to improve soybean oil content and quality.

References:

［1］Panthee D R, Pantalone V R, West D R, et al. Quantitative trait loci for seed protein and oil concentration, and seed size in soybean［J］. Crop Science, 2005, 45(5):2015-2022

［2］Fehr W R. Breeding for modified fatty acid composition in soybean［J］.Crop Science, 2007, 47(Supplement_3): S-72-S-87
［3］Rose D P.Effects of dietary fatty acids on breast and prostate cancers: evidence from in vitro experiments and animal studies［J］.The American Journal of Clinical Nutrition, 1997, 66(6): 1513S-1522S
［4］Demaison L, Moreau D. Dietary n-3 polyunsaturated fatty acids and coronary heart disease-related mortality: a possible mechanism of action［J］. Cellular and Molecular Life Sciences CMLS, 2002, 59(3): 463-477
［5］Burdge G C, Calder P C.Conversion of alpha-linolenic acid to longer-chain polyunsaturated fatty acids in human adults［J］.Reproduction Nutrition Development, 2005, 45(5): 581-598
［6］XiaY F. Study on the development of China soybean industry［M］.Beijing:Chinese Merchandise Press, 2003: 1-25
［7］Luan X, Man W, Chen Y, et al.Achievement and prospect of soybean breeding in Heilongjiang Province from 1986-2000［J］. Soybean Science, 2003, 23(2): 134-142
［8］Xu D H, Gai J Y. Genetic diversity of wild and cultivated soybeans growing in China revealed by RAPD analysis［J］. Plant Breeding, 2003, 122(6): 503-506
［9］Pham A T, Lee J D, Shannon J G, et al.Mutant alleles of FAD2-1A and FAD2-1B combine to produce soybeans with the high oleic acid seed oil trait［J］.BMC Plant Biology, 2010, 10(1): 195［10］D VAZIRI N, Kim C H, Phan D, et al. Up-regulation of hepatic Acyl CoA: diacylglycerol acyltransferase-1 (DGAT-1) expression in nephrotic syndrome［J］.Kidney International, 2004, 66(1): 262-267.
［11］Pulsifer I P, Kluge S, Rowland O. Arabidopsis long-chain acyl-CoA synthetase 1 (LACS1), LACS2, and LACS3 facilitate fatty acid uptake in yeast［J］. Plant Physiology and Biochemistry, 2012, 51: 31-39
［12］Qi Q, Huang Y, Cutler A J, et al. Molecular and biochemical characterization of an aminoalcoholphosphotransferase (AAPT1) from Brassica napus: effects of low temperature and abscisic acid treatments on AAPT expression in Arabidopsis plants and effects of over-expression of BnAAPT1 in transgenic Arabidopsis［J］. Planta, 2003, 217(4): 547-558
［13］Song Q X, Li Q T, Liu Y F, et al. Soybean GmbZIP123 gene enhances lipid content in the seeds of transgenic Arabidopsis plants［J］.Journal of Experimental Botany, 2013: ert238
［14］Ralston A W. Fatty acids and their derivatives［J］.Fatty Acids and Their Derivatives, 1948
［15］Hori K, Watanabe Y.UPF3 suppresses aberrant spliced mRNA in Arabidopsis［J］. The Plant Journal, 2005, 43(4): 530-540.
［16］Ramsey A J, Chinkers M. Identification of potential physiological activators of protein phosphatase 5［J］. Biochemistry, 2002, 41(17): 5625-5632
［17］Chen M X, Cohen P T W.Activation of protein phosphatase 5 by limited proteolysis or the binding of polyunsaturated fatty acids to the TPR domain［J］. FEBS Letters, 1997, 400(1): 136-140
［18］盖钧镒, 汪越胜.中国大豆品种生态区域划分的研究［J］. 中国农业科学, 2001, 34(2): 139-145.（Gai J Y, Wang Y S. A study on the varietal eco-regions of soybeans in China［J］. Chinese Agricultural Sciences, 2001, 34(2): 139-145.）
［19］Song Q X, Li Q T, Liu Y F, et al. Soybean GmbZIP123 gene enhances lipid content in the seeds of transgenic Arabidopsis plants［J］.Journal of Experimental Botany, 2013: ert238
［20］Porebski S, Bailey L G, Baum B R.Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components［J］. Plant Molecular Biology Reporter, 1997, 15(1): 8-15.

Memo

Memo:
-
Last Update: 2015-12-30