|Table of Contents|

Optimization of Soybean Embryonic Tip Regeneration System and Transferring cry1C*?Gene into Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年05期
Page:
910-913,917
Research Field:
Publishing date:

Info

Title:
Optimization of Soybean Embryonic Tip Regeneration System and Transferring cry1C*?Gene into Soybean
Author(s):
JIA Bao-hui LI NaYU Xi-sen LI Han-zhe LI Yang LIU Yu-ting MENG Fan-li LI Wen-bin
Agronomy College of Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, China
Keywords:
Soybean(Glycine max L) Embryonic tip Genetic transformation cry1C*
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.05.0910
Abstract:
Abstract: Through the optimization of shoot induction medium and shoot elongation medium, established a embryonic tips transformation system with embryonic tips of Dongnong 8004 and transformed cry1C* gene into soybean by this system. The resistant plants were identified by molecular and insect resistance methods. The results showed that the percentage of shoots induced reached 80% in shoot induction medium with 1.67 mg.L-16-BA and the rate of shoot elongation reached 54% in shoot elongation medium with 0.5 mg?L-1?GA3?and 0.1 mg,L-1?IAA. A total of 94 resistant plants with 100 μg.mL-1PPT were determined by PCR, which included 14 positive lines of transformation and the rate was 14.89%.We found that the cry1C* gene expressed in soybean which could improve the resistance in pod borer. After the resistant identification of soybean pod borer with T0?positive plants, we discovered that the resistance of C-1 was higher than Dongnong 8004.

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Last Update: 2015-11-09