|Table of Contents|

Internal Transcribed Spacer(ITS) sequence identification of rDNA in a Pythium strain PSHH1(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年05期
Page:
867-873
Research Field:
Publishing date:

Info

Title:
Internal Transcribed Spacer(ITS) sequence identification of rDNA in a Pythium strain PSHH1
Author(s):
ZHAN Grui-ping1; WANG Jia-jun1; WEI Lai1; ZHANG Wu2; LUAN Xiao-yan1; TANG Xiao-fei1; LIU Xin-lei1
1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;
2.Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, China
Keywords:
Pythium;  Internal Transcribed Spacer(ITS) sequence identification;  Pathogenicity
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.05.0867
Abstract:
Pythium is an important part of soybean root rot fungal population in Heilongjiang province, in order to determine the species of Pythium in Heilongjiang province, PSHH1 has been isolated from the soil of soybean root in Heihe city. And Rdna-ITS sequence analysis was applied to PSHH1。According to the results of the analysis of rDNA-ITS sequences, the sequence homology was more than 99% when it was compared with the sequence of registration number AB259316.1 and AB512973.1 in GeneBank.Therefore, we think PSHH1 was a Pythium sylvaticum. In order to study the pathogenicity of PSHH1, using seed rot method and hypocotyl inoculation method, 60 main soybean cultivars were selected to determine the pathogenicity of PSHH1, and seed rot rate and stem discount rate can be reflected to evaluate the pathogenicity of PSHH1,results showed that PSHH1 had strong pathogenicity to 13.3% main soybean cultivars, at the same time, there were 61.67% main soybean varieties expressed weak pathogenicity to PSHH1。

References:

［1］白丽艳.大豆根腐病病原种类鉴定及分子检测技术研究［D］.乌鲁木齐:新疆农业大学,2009.(Bai L Y. Study on pathogen identification and molecular detection technology of soybean root rot［J］.Urumuchi:Xinjiang Agricultural University,2009.)

［2］李保英,马淑梅.大豆根腐病原菌种类及抗源筛选［J］植物保护学报,2000,27(1):91-93.(Li B Y, Ma S M。Pathogens of soybean root rot and screening of resisrant source［J］.Acta Phytophylacica Sinica,2000,27(1):91-93.)
［3］银玲,田讯,霍万学.腐霉属菌形态学及分子生物学鉴定技术概述［J］.中国蔬菜,2013(12):15-22.(Yin L,Tian X,Huo W F. Overview of identification techniques for Pythium species based on morphology and molecular biology［J］.China Vegetables,2013(12):15-22.)
［4］马淑梅.黑龙江省大豆根腐病致病病原种类分布及抗病种质鉴定［J］.中国农学通报,2012,28(27):230-235.(Ma S M.Pathogenic pathogen categories distribution and germplasm resistance identification of soybean root rot in Heilongjiang province［J］.Chinese Agricultural Science Bulletin,2012,28(27):230-235)
［5］Ho H H.Thegenus Pythium inmainl and China［J］. Mycosystema,2013,32(S1):20-44
［6］李金花,柴兆祥.甘肃腐霉新记录种Pythium carolinianum的分离鉴定及rDNA-ITS序列分析［J］.兰州大学学报（自然科学版）,2010, 46 (6):84-95.(Li J H and Cai Z X.A new recrd of Pythium carlinianum in Gansu province and its isolation,identificatin and rDNA-ITS sequence analysis［J］Journal of Lanzhou University(Natural Sciences),2010,46(6):84-95.)
［7］朱振东.大豆疫霉根腐病的发生和防治研究进展［J］.植保技术与推广,2002,22(7):40-42.(Zhu Z D.Progression of Phytophthora root rot of soybean research and prevention［J］Plant Protection and Extension2002,22(7):40-42.)
［8］楼兵干,张炳欣.一种适合于从植物病组织中分离腐霉菌的选择性培养基［J］.杭州电子工业学院学报,2000,20(3):72-74(Lou B G，Zhang B X.A medium selective for the isolation of pythium species from diseased plant tissues［J］.Journal of Hangzhou Institute of Electronic Engineering,2000,20(3):72-74.)
［9］柴兆祥,李金花,楼兵干,等。玉米根围土壤中腐霉菌的分离鉴定及核糖体DNA-ITS序列分析［J］.草业学报,2009,18(3):126-135.(Chai Z X,Li J H,Lou B G, et al. Isolation, identification, and sequence analysis of rDNA-ITS of Pythium species sampled from rhizosphere soil of corn［J］. Acta Prataculturae Sinica,2009,18(3):126-135.)
［10］方忠达.植病研究方法［M］.北京:中国农业出版社,1998:11-65.(Fang Z D. Research methods of plant disease［M］.Beijing:China Agriculture Press,1998:11-65.)
［11］Broders K D,Lipps P E,Paul P A,et al.Chracterization of Pythium spp.associated with corn and soybean seed and seedling disease in Ohio［J］.Plant Disease,2007,91:727-735
［12］Zhang B Q,Yang X B.Pathogenicity of Pythium populations from corn-soybean rot ation fields［J］.Plant Disease,2000,84:94-99
［13］魏崃,李文滨,韩英鹏,等.大豆品种对四种腐霉菌的抗性研究［J］.大豆科学,2010,29(6):971-975.(Wei L,Li W B,Han Y P, et al.Identification of esistance to four Pythium species in soybean cultivars［J］.Soybean Science,2010,29(6):971-975.)
［14］于洪久,朱洪德,刘丽君,等. 腐霉菌侵染条件下大豆下胚轴中PAL和POD活性的变化［J］.大豆科学,2012,31(3):495-497.（Yu H J, Zhu H D, Liu L J, et al.PAL and POD actioities of soybean hypocotyls infected by Pythium［J］.Soybean Science,2012,31(3):495-497.）
［15］Shehata M A.Response of susceptible and moderately resistant pea genotypes to interaction between Rhizoctonia solani and three other stem and root rot pathgens［J］.Plant Disease,1983,67(10):1146-1148.
［16］Wang X Y,Wen J Z. Species and pathogenicity of Fusarium causing soybean root rot in Northeast China［J］ Chinese Journal of Oil Crop Sciences,2011,33(4):391-395.
［17］马淑梅.黑龙江省大豆根腐病致病病原种类分布及抗病种质鉴定［J］.中国农学通报,2012,28(27):230-235.(Ma S M. Pathogenic pathogen categories distribution and germplasm resistance identification of soybean root rot in Heilongjiang province［J］. Chinese Agricultural Science Bulletin,2012,28(27):230-235.)
［18］张丽.黑龙江省大豆尖镰孢抗源筛选、致病机理及遗传多样性分析［D］.哈尔滨:东北农业大学,2014.(Zhang L.Resistant materials screening, pathogenic mechanism and genetic diversity analysis of Fusarium Oxysporum causing soybean root rot in Heilongjiang province［D］.Harbin: Northeast Agricultural University, 2014.)
［19］陈秀贤,曾会才,Ho H H,等.分子生物学技术在腐霉菌分类上的应用研究［J］.生物技术通报,2007(5):84-92.(Chen X X, Zeng H C, Ho H H, et al. Application of molecular biology on taxonomy of Pythium［J］.Biotechnology Bulletin,2007(5):84-92.)
［20］林剑伟,阙友雄,陈天生,等.核糖体DNA的内转录间隔区序列标记在真菌分类鉴定中的应用［J］.生物技术通讯,2007,18(2):292-294.(Lin J W,Que Y X,Chen T S,et al.Application of ribosomal DNA internal transcribed spacer in Fungi taxonomy［J］.Letters in Biotechnology,2007,18(2):292-294.)
［21］陈凤毛。真菌ITS区序列结构及其应用［J］.林业科技开发,2007,21(2):5-7.(Chen F M. Fungal sequences of ITS structure and its application［J］. China Forestry Science and Technology,2007,21(2):5-7.)
［22］Briard M, Dutetre M, Rouxel F, et al. Ribosomal RNA sequence divergence within the Pythiaceae［J］Mycological Research,1995,99:1119-1127.
［23］Wang P H, White J G. Molecular characterization of Pythium species based on RFLP analysis of the internal transcribed spacer region of ribosomal DNA［J］.Physiological Molecular Plant Pathology,1997,51:129-143.
［24］Mastumoto C,Kageyama K,Suga H, et al.Phylogenetic relationships of Pythium species based on ITS and 5.8S sequences of the ribosomal DNA［J］Mycoscience1999,40:321-331
［25］Martin F N.Phylogenetics among some Pythium species inferred from sequence analysis of the mitochondrially encodedcy to chrome oxidaseⅡgene［J］.Mycologia,2000,92:711-727
［26］楼兵干,张炳欣.基于rDNA ITS序列探讨部分腐霉种的系统发育与其形态特征［J］.菌物学报,2005,24(2):207-220(Lou B G, Zhang B X.Phylogenetic relationships and ITS morphological characteristics among some Pythium species inferred from sequence analysis of rDNA ITS［J］. Mycosystema,2005,24(2):207-220.)
［27］边传红,王伟杰,赵世民,等.烟草钟器腐霉的分子鉴定与致病力测定［J］.农学学报,2015,5(1):32-36.(Bian C H,Wang W J,Zhao S M,et al. Molecular identification and pathogenicity of Pythium vexans isolated from tobacco［J］.Journal of Agriculture,2015,5(1):32-36.)
［28］Bates G D,Rothrock C S,Rupe J C.Resistance of the soybean cultivar Archer to Pythium damping-off and root rot caused by several Pythium spp［J］.Plant Disease,2008,92:763-766

Memo

Memo:
-
Last Update: 2015-11-08