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Behaviors of Protein Storage Vacuoles and Oil Bodies during Soybean Germination by Transmission Electron Microscopy(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年05期
Page:
833-836,842
Research Field:
Publishing date:

Info

Title:
Behaviors of Protein Storage Vacuoles and Oil Bodies during Soybean Germination by Transmission Electron Microscopy
Author(s):
GUO Jin-yan;  ZHAO Lu-ping;  CHEN Ye-ming;  HUA Yu-fei
School of Food Science and Technology/State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Keywords:
Soybean germination;  Oil bodies;  Protein storage vacuoles;  Oleosin hydrolysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.05.0833
Abstract:
Soybean germination is not only used to produce soybean sprouts, but also important in soybean growth.The protein in protein storage vacuoles (PSVs) and the lipid in oil bodies (OBs) are hydrolyzed in soybean germination to supply materials and energy for growth and seedling.In this study, transmission electron microscopy was used to monitor the behaviors of OBs and PSVs in soybean germination. It was found that each soybean cell was fully filled with OBs and PSVs in dry soybean. During germination, PSVs were gradually coalesced into larger vacuoles, and formed one final vacuole after 4 days, and the contents of PSVs were gradually decreased; after 1-2 days germination, OBs tended to approach the PSVs, and the approached OBs were smaller than the ones far from PSVs; most of OBs was mobilized to become smaller ones or disappear, and a few of OBs were coalesced into large oil droplets, which should be closely correlated with the oleosin hydrolysis. On one hand, oleosin hydrolysis could expose the triglycerides, which was beneficial for the subsequent hydrolysis of triglycerides.On the other hand, oleosin could efficiently inhibit the coalescence of OBs, so oleosin hydrolysis should be the reason for the formation of large oil droplets. At last, it was found that the mobilization of PSVs, especially OBs, was quicker when soybean germination was conducted with light.

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Last Update: 2015-11-08