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Effects of Light Spectra on Soybean Cotyledonary Node Regeneration System(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年05期
Page:
826-832
Research Field:
Publishing date:

Info

Title:
Effects of Light Spectra on Soybean Cotyledonary Node Regeneration System
Author(s):
WEN Tao;  JIAO Xue-lei;  LIU Xiao-ying;  XU Zhing-gang
College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
Keywords:
Soybean;  Light spectra;  Cotyledonary node;  Multiple shoot;  LED light
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.05.0826
Abstract:
The soybean cultivar Liaoxian 1 was used as the experimental material to determine the effects of different light spectra on soybean cotyledonary node regeneration. Under six different light conditions, the soybean cotyledonary nodes from germinated seeds for 7 d were used as the explant and inoculated onto shoot initiation, shoot elongation and rooting medium successively. The photosynthetic photon flux density (PPFD) was 50 μmol.m-2.s-1, and photoperiod was 12 h light/12 h dark.The results showed that the 630 nm red light resulted in inhibition of elongation between multiple shoots.Compared with 630 nm red light, 660 nm red light promoted remission of this inhibition noticeably, and resulted in greater growth, chorophyll accumulation and dry mass of multiple shoots, and greater growth, dry mass and root development of regenerated plantlet. Green or yellow light added to the complex spectra of red and blue light resulted in inhibition of elongation between multiple shoots, yellow light aggravated this inhibition. Green light added to the complex spectra of red and blue light resulted in greater growth, chorophyll accumulation and dry mass of multiple shoots, and rooting of soybean plantlet in vitro

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Last Update: 2015-11-07