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Title:: Clone and Sequence Analysis of Virulence Genes of Soybean Mosaic Virus Isolates in China

Author(s):: WANG Da-gang¹; 2; ZHI Hai-jian²; TIAN Zhen²; HUANG Zhi-ping¹; WU Qian¹; ZHANG Lei¹; 1.Crop Institute of Anhui Academy of Agricultural Sciences/Key Laboratory of Crop Quality Improvement of Anhui Province, Hefei 230031, China;
2.Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement,Nanjing 210095, China

Keywords:: Soybean mosaic virus; Virulent genes; Clone; Sequences analysis

PACS:: -

DOI:: 10.11861/j.issn.1000-9841.2015.05.0760

Abstract:: Five SMV isolates were obtained by using biological purification and serology (DAS-ELISA).The CP,HC-Pro,P1 and P3 gene regions were amplified by reverse transcription polymerase chain reaction (RT-PCR)Sequences analysis showed that P1 gene of the each SMV isolates had 927 nucleotides in length, encoding 309 amino acids.Comparison of the sequences of P1 genes between different isolates showed they shared 88.0%-99.9% nucleotide acids identities and 86.1%-100.0% amino acids identities with each other. The four genes from the 5 SMV isolates were composed of 4 137 nucleotides, encoding 1 378amino acids, and shared 92.6%-99.3% nucleotide sequence identities and 95.1%-99.2% amino acid sequence identities. The results also revealed that the sequences variation in the CP,HC-Pro,P1 and P3 genes of virus were correlated with the level of virulence and the source of the virus isolates based on the symptom reaction of SMV on differential hosts and the phylogenetic analyses .Meanwhile, the normal soybean mosaic virus isolates and recombinant isolates could be differentiated through the sequences variation of CP,HC-Pro,P1 and P3genes.

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Clone and Sequence Analysis of Virulence Genes of Soybean Mosaic Virus Isolates in China(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

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