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Title:: Identification of miRNA Resistant to Phytophthora Root Rot in Soybean

Author(s):: GUO Na¹; CUI Xiao-xia¹; ZHAO Jin-ming¹; YAN Qiang²; WU Xiao-ling¹; DOU Dao-long²; XING Han¹; 1.Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China; 2.Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China

Keywords:: Glycine max; Conserved miRNAs; Northern blotting; Phytophthora root and stem rot

PACS:: -

DOI:: 10.11861/j.issn.1000-9841.2015.04.0666

Abstract:: The microRNAs (miRNAs), a type of short (20-24 nucleotides), non-coding RNA molecules, mediate repressive gene expression through RNA silencing at the transcriptional and post-transcriptional level, and play an important role in the regulation of plant growth and development, and defense and response to various abiotic and biotic stresses.Phytophthor a root and stem rot (PRR) of soybean caused by Phytophthora sojae is a destructive disease affecting soybean production regions throughout the world.However, the miRNAs-regulation networks and function in response to PRR are not clear in Glycine max. In this paper, seven conserved miRNAs were detected in eight cultivars of pre and post-inoculation by Northern blotting.The results showed that, six conserved miRNAs were expressed in eight cultivars in pre and post-inoculation, but there is no significant difference in pre-and post-inoculation Furthermore, we used microarray to detect miRNA expression pattern. The result showed that some miRNAs play very important roles in response to PRR in Glycin e max.

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Identification of miRNA Resistant to Phytophthora Root Rot in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

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