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Effects of N+Implantation on Germination Percentage of Seeds and Physiological Property of Seedlings in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年04期
Page:
630-634
Research Field:
Publishing date:

Info

Title:
Effects of N+Implantation on Germination Percentage of Seeds and Physiological Property of Seedlings in Soybean
Author(s):
ZHANG Hong12 ZHENG Shi-ying1 LIU Gui-zhong23 SU Rong-cun1 LIANG Shu-xia1 Xiao Bei-lei1 GENG Jian-fen1
1.College of Ecology and Garden Architecture, Dezhou University, Dezhou 253023, China;?
2.Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics, Dezhou University, Dezhou 253023, China;?
3.College of Physics and Electronic Engineering, Dezhou University, Dezhou 253023, China
Keywords:
Soybean N+?implantation Germination percentage of seeds Physiological property
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.04.0630
Abstract:
In order to reveal the intrinsic relationships between changing rule of the seedling physiological and biochemical indexes and N+?implantation energy and dose, exploring suitable N+implantation energy and dose for different soybean varieties, effects of N+?implantation on percentage of seeds, activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT),and contents of malondialdehyde(MDA)and soluble protein physiological indexes were researched using four soybean cultivars,namely Qihuang 34(Q34),Dedou 99-16(D99-16),Jidou 12(J12),and Hedou 12(H12),as materials and 6 treatments (15 keV,2.4×1013N+.m-2;15 keV,4.8×1013+.m-2;15 keV,7.2×1013N+.m-2;25 keV,2.4×1013N+.m-2;25 keV,4.8×1013N+.m-2;25 keV,7.2×1013N+.m-2).The results were as follows: In a certain range of energy and dosage, with the increase of the implantation energy and dose, the germination rate of seeds, the activities of SOD, POD and CAT, and soluble protein content all expressed a change trend of first increasing then dropping, and the tendency of the MDA content was on the contrary. Suitable values of energy and dose for N+implantation, promoting the seedling growth of various varieties, were D99-16 and J12: 15 keV, 4.8 × 1013 N+.m-2; H12: 15 keV, 7.2 × 1013 N+.m-2; Q34: 25 keV, 2.4 × 1013N+.m-2; respectively Appropriate values for mutation breeding were J12, H12, and D99.16: 25 keV, 2.4× 1013N+.m-2; Q34: bigger than 25 keV, 7.2×1013 N+.m-2. The research was significant for soybean mutation breeding by N+implantation method.

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Last Update: 2015-09-01