|Table of Contents|

Analysis on the Candidate Resistance Gene GmZ15 to Soybean Mosaic Virus by VIGS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年04期
Page:
582-587
Research Field:
Publishing date:

Info

Title:
Analysis on the Candidate Resistance Gene GmZ15 to Soybean Mosaic Virus by VIGS
Author(s):
HUANG Sai-hua ZHENG Gui-jie YANG Yong-qing ZHI Hai-jian
Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China
Keywords:
Soybean mosaic virus(SMV) Virus induced gene silencing Resistance gene Function analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.04.0582
Abstract:
Soybean mosaic virus(SMV) is one of the most prevalent pathogens in soybean production in China.To clarify the mechanism for SMV resistance would be great significant VIGS (virus induced gene silence) was used to analyze the functions of candidate gene GmZ15 for resistance locus RSC3Q.The results showed the expression of GmZ15 changed greatly after inoculated with SMV, and reached a peak 24 hours later. After GmZ15 was silenced, the expression of GmZ15 dropped to 0-3 compared with the control inoculated with buffer and 0-2 compared with the control inoculated with empty vector pGG7R2. The silencing of GmZ15 contributes to the accumulation of SMV in soybean. The accumulation of SMV was 6 times more in soybean which GmZ15 was silenced than in the control.While, there was no significant difference in the upper leaf. So we speculated that GmZ15 did induce a kind of defense reaction.There may be other gene(s) involved in the resistance process in Qihuang 1.

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Last Update: 2015-08-31