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Agrobacterium-mediated GmDAD1 Gene Silence of Transgenic Hairy Roots in Chimeric Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年04期
Page:
576-581
Research Field:
Publishing date:

Info

Title:
Agrobacterium-mediated GmDAD1 Gene Silence of Transgenic Hairy Roots in Chimeric Soybean
Author(s):
XU Na1;  YAN Qiang1; 2;  CUI Xiao-xia1; GUO Na1;  DOU Dao-long2;  XING Han1
1Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement /Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture/State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China; 2Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China
Keywords:
Soybean; Hairy root; Chimeric plant; A rhizogenes; GmDAD
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.04.0576
Abstract:
Agrobacterium rhizogenes is able to transform plant genomes and induce the production of hairy roots.In this work, we described the use of Arhizogenes in soybean to rapidly assess gene expression and function. We first cloned the soybean –DAD- gene into pHellsgate12:GFP vector, which was reconstructed by inserted a 35S:GFP:nos expression cassette into SacI digested pHellsGate12, then transformed the DAD-gene fragment into soybean by using this chimeric hairy root transgenic system.The transcription level of DAD in the fluorescence hairy roots were reduced to 45%~70%.The results revealed that the DAD gene was silenced in transgenic hairy roots, and the reconstructed RNAi vector could simplify the progress of positive hairy roots screen.

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Last Update: 2015-08-31