|Table of Contents|

SEM Observation after Extraction of Epicuticular Wax on Soybean Leaves(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年03期
Page:
540-544
Research Field:
Publishing date:

Info

Title:
SEM Observation after Extraction of Epicuticular Wax on Soybean Leaves
Author(s):
LI Na WANG Li-naJIN Xun RUI Hai-ying PAN Hong-li JIN Ling LI Ze-yu YU Ji-dong
Daqing Branch of Heilongjiang Academy of Agricultural Sciences,Daqing 163316,China
Keywords:
SoybeanEpicuticular waxChloroformn hexaneSEM
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.03.0540
Abstract:
Extraction of epicuticular wax on mature soybean leaves(Kangxian 11 and Heinong 48) are studied comparatively with solution CHCl3and solution n-hexane with room-temperature method and high-temperature method.The microstructure observed by scanning electronic microscope (SEM) showed that high-temperature method was better than room-temperature method. Epicuticular wax was not completely extracted with room temperature method with both solution CHCl3?and solution n-hexane, there was still a small amount of flocculent or granular waxy residue, but the extraction effect of chloroform was better than n-hexane. Epicuticular wax was completely extracted with high-temperature method with both solution CHCl3?and solution n-hexane, extraction time was found significantly affecting the extracted wax quality, high-temperature treatment 30 s, leaf epidermal cells had undergone varying degrees of deformation.In the present study, 60℃, 15 s with solution CHCl3?was the effective and simple extraction method for epicuticular wax on soybean leaves by SEM.

References:

[1]Eglinton G,Hamilton R J. Leaf epicuticular waxes[J]. Science,1967,156: 1322-1335

[2]Schreiber L, Skrabs M, Hartmann K D, et al.Effect of humidity on cuticular water permeability of isolated cuticular membranes and leaf disks[J]. Planta,2001,214(2):274-282
[3]Kunst L,Samuels A L.Biosynthesis and secretion of plant cuticular wax[J]. Progress in Lipid Research.2003,42(1):51-80
[4]李婧婧,黄俊华,谢树成.植物蜡质及其与环境的关系[J].生态学报,2011, 31(2): 565-574(Li J J,Huang J H, Xie S H.Plant wax and its response to environmental conditions and overview[J]. Acta Ecologica Sinica, 2011, 31(2): 565-574)
[5]Xu S J, Jiang P A,Wang Z W, et al.Crystal structures and chemical composition of leaf surface wax depositions on the desert moss syntrichia caninervis[J]. Biochemical Systematics and Ecology,2009,37(6):723-730
[6]Gonzalez A,Ayerbe L.Effect of terminal water stress on leaf epicuticular wax load,residual transpiration and grain yield in barley[J].Euphytica,2010,172(3):341-349
[7]顾俊,王飞,张鹏,等.植物叶表皮蜡质的生物学功能[J].江苏农业学报,2007,23(2):144-148(Gu J,Wang F,Zhang P,et al.Biological function of epicuticular wax[J]. Jiangsu Journal of Agricultural Sciences, 2007,23(2):144-148)
[8]李灵之,马杰,向建华,等.植物角质层内外蜡质的差异及其与抗逆性的关系[J]植物生理学报,2011,47 (7): 680-684(Li L Z, Ma J, Xiang J H, et al.Composition differences of epicuticular and intracuticular wax layers and the relationship between cuticle and plant stress tolerance[J]. Plant Physiology Journal,2011,47 (7): 680-684)
[9]姬生栋,王育水,贾振杰,等.不同产量水平小麦叶片表面超微结构初探[J].河南农业科学,2003(9): 4-7(Ji S D,Wang Y S, Jia Z J, et al. Structure preliminary study on blade surface ultramicroscopic of spring wheat with different yield level[J]. Henan Agricultural Sciences,2003(9): 4-7)
[10]吴杏春,林文雄,黄忠良.UV2B辐射增强对两种不同抗性水稻叶片光合生理及超显微结构的影响[J]生态学报,2007,27(2):554-563.(Wu X C,Lin W X,Huang Z L. Influence of enhanced ultraviolet 2B radiation on photosynthetic physiologies and ultrastructure of leaves in two different resistivity rice cultivars[J].Acta Ecologica Sinica,2007,27(2):554-563)
[11]邵云,姜丽娜,李万昌,等.砷、铅胁迫对小麦幼苗毒害效应及叶片下表皮扫描电镜观察[J]西北农业学报,2009,18(1): 133-138(Shao Y, Jiang L N,Li W C, et al.Toxic effects of as and Pb to wheat seedling and scanning electron-microscopic observation on nether epidermis of leaves[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(1):133-138)
[12]高臣,刘俊渤,常海波,等.硅对水稻叶片光合特性和超微结构的影响[J].吉林农业大学学报,2011,33(1):1-4(Gao C,Liu J B,Chang H B, et al.Effects of silicon on rice leaf photosynthesis and ultrastructure[J]. Journal of Jilin Agricultural University,2011,33(1):1-4)
[13]Kwan Su Kim, Si Hyung Park, Dong Kwan Kim, et al.Influence of water deficit on leaf cuticular waxes of soybean(Glycine Max[L] Merr)[J]. International Journal of Plant Sciences,2007,168(3):307-316
[14]周小云,陈信波,徐向丽,等.稻叶表皮蜡质提取方法及含量的比较[J].湖南农业大学学报(自然科学版),2007,33(3):273-276(Zhou X Y,Chen X B,Xu X L, et al.On comparison of extraction methods of epicuticular wax and content of rice leaves[J].Journal of Hunan Agricultural University (Natural Sciences),2007,33(3):273-276)
[15]王学东,崔琳,向文胜.植物叶片表面蜡质除去方法的研究[J].东北农业大学学报,1999,30(4):412-414(Wang X D,Cui L,Xiang W S,Studies method for the remdval plants leaves surface waxiness[J]. Journal of Northeast Agricultural University, 1999, 30(4):412-414)
[16]杨艳青.苹果果实表皮蜡质结构观察与组分分析[D]. 杨凌:西北农林科技大学,2013(Yang Y Q.The structure observation and component analysis of apple fruit epicuticular wax[D]. Yangling:Northwest Agricultural and Forestry University,2013)

Memo

Memo:
-
Last Update: 2015-07-16