Comparative Study of Two Methods for Discrimination of Homonymous Soybean Varieties(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2015年03期

Page:

530-535

Research Field:

Publishing date:

Info

Title:

Comparative Study of Two Methods for Discrimination of Homonymous Soybean Varieties

Author(s):

ZHAO Jing¹; 2;  HE Juan-juan²;  XIN Xia²;  LU Xin-xiong²;  ZHOU Yan-lin¹

1.School of Life Sciences, Inner Mongolia University, Hohhot 010021,China;

?2. National Genebank of China, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Keywords:

Homonym;  Soybean;  Mancangjin;  Electronic nose;  PCA;  Agro-traits

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2015.03.0530

Abstract:

Crop varieties with the same name brings a big challenge to genebank management, as genebank managers should secure that every variety being kept in the genebank is unique. In the present work, we tested the possibilities of variety discrimination by a non-destructive manner using electronic nose. The tested materials were 10 soybean varieties with the same name ‘Mancangjin’ selected from the National Genebank of China.Their code was ZDD0376，ZDD0604，ZDD0605，ZDD0606，ZDD0841，ZDD0078，ZDD0607，ZDD0925，ZDD1013 and ZDD1077, 5 of them with high genetic similarity coefficient, the other 5 with low genetic similarity coefficient.The head-space volatiles of these varieties were tested by a PEN3 electronic nose, and the signals were analyzed using principal component analysis (PCA) and linear discriminant analysis (LDA).The 10 varieties were also discriminated by documentation agro-traits.Agro-traits clustering analysis showed that using 13 agro-traits could only distinguish 6 varietiesVarieties ZDD604 and ZDD607, ZDD605 and ZDD606 were clustered together, as there were 8 and 10 agro-traits were completely consistent with each other, respectively. So it was difficult to distinguish these two groups by documentation agro-traits.The electronic nose signals that analyzed by LDA method could only divided the 10 varieties into 6 categories ZDD0604,ZDD0605,ZDD0606 and ZDD0841 which with high genetic similarity coefficient were difficult to distinguish, yet ZDD0078,ZDD0607,ZDD0925 and ZDD1077 which with low genetic similarity coefficient were easy to distinguish.In addition, ZDD0376 and ZDD1013 were also difficult to distinguish. While using PCA method could distinguish all those 10 varieties clearly. Two varieties were selected randomly which the code were ZDD0376 and ZDD1077.They were further used to verify the validity of electronic nose technology for identification of soybean varieties by PCA method.The accuracy of the results was high, declaring that the use of electronic nose-PCA technique is reliable in the identification of homonymous soybean varieties.

References:

［1］卢新雄, 曹永生. 作物种质资源保存现状与展望［J］.中国农业科技导报, 2001, 3(3):43-47 (Lu X X, Cao Y S.Current status and prospect of crop germplasm resources for ex situ conservation［J］.Review of China Agricultural Science and Technology, 2001, 3(3):43-47)

Memo

Memo:

-

Common functions