|Table of Contents|

Residue Analysis Method of Imazethapyr in Soybean Plants, Seed and Soybean Field by QuEChERS-HPLC(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年03期
Page:
480-484
Research Field:
Publishing date:

Info

Title:
Residue Analysis Method of Imazethapyr in Soybean Plants, Seed and Soybean Field by QuEChERS-HPLC
Author(s):
LI Guang-ling;  DONG Li-hong;  LIU Bo;  GUO Wen-ju;  CHEN Xi-ling
Henan Institute of Science and Technology, Xinxiang 453003, China.
Keywords:
Imazethapyr;  Soybean plant;  Soybean seed;  Soybean field soil;  Dispersive solid phase extraction;  Residue analysis.
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.03.0480
Abstract:
A method based on dispersive solid phase extraction determining Imazethapyr residue in soybean plants, seed and soybean field by HPLC-UVD was developed.The target analysis was quantified by external standard method and matrix-matched standard solution used to compensate for matrix effect. The sample was extracted by methanol and cleaned up by dispersive solid phase extraction using primary secondary amine(PSA) and C18-bonded silica dispersant sorbent. Under the optimal conditions, the retention time of Imazethapyr was 697 min, the calibration curves showed good linearity(y=4.003 8x-0.275 6, R2=0.999 5）from 0.01 to 10.0 mg?L-1concentration of Imazethapyr, the average recoveries were determined in soybean plants, soybean and soybean field blank spiked at 0.05, 0.1 and 0.5 mg?kg-1?and the overall recovery rates were over 85%, with the relative standard deviations(RSD) less than 9%.The detection limit of Imazethapyr was 0.104 ng, and the limit of quantification was 0.024, 0.033 and 0.026 mg?kg-1in soybean plants, seed and soybean field respectively. The results indicated that the sample pretreatment method and the residue sample detection method were simple, good effect, low operation cost, and stable reliability.

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Last Update: 2015-07-16