Identification of Pathogen Causing Bacterial Pustule Spot of Soybean and Resistance Evaluation of New Soybean Germplasm(PDF)

¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2015Äê03ÆÚ

Page:

463-469

Research Field:

Publishing date:

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Title:

Identification of Pathogen Causing Bacterial Pustule Spot of Soybean and Resistance Evaluation of New Soybean Germplasm

Author(s):

ª¤XU Yuan¹; CHENG Wei²; WU Hui-jun¹; ZHENG Lei-qi¹; ZHAO Tuan-jie²; GAO Xue-wen¹

1.College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Pest Management in Crops in Eastern China, Ministry of Agriculture, Nanjing 210995, China;

?2.National Center for Soybean Improvement/ National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China

Keywords:

Soybean;  Xanthomonas axonopodis pv-Glycines;  Pathogen identification;  Resistant gemplasm

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2015.03.0463

Abstract:

In this study, the pathogen which caused the serious disease of soybean leaves in Jiangsu province was isolated and identified, and a set of newly developed varieties, breeding lines and parents were used to evaluate the disease resistance to the pathogen. The results showed that four strains, which were isolated from different soybean leaf samples could result in the same symptom, were identified as the pathogen.Then, through morphology, biochemical and physiological identification, and 16S rDNA and gyrB cluster analysis, those 4 strains were further classed as the Xanthomonas axonopodis pv-glycines. Different resistance reactions of soybean germplasm to C5 and B523 pathogen strains could be observed. The ratios of soybean accessions with resistance to C5 and B523 were only 40.35% and 37.77%, respectively. It indicated that the resistance level of these soybean lines was not high, and there was precondition for widespreading of the disease. Among the 233 tested accessions, two soybean varieties, Suxian 21 and Xudou 16, showed high resistance to the two strains, and could be used for developing disease-resistant varieties.

References:

£Û1£ÝÕŽð÷,Îé»Ô¾ü,¸ßѧÎÄ.¶¡Ïã¼Ùµ¥°û´ó¶¹Ö²¡±äÖÖÁ½¸öIIIÐÍЧӦÒò×ӵĿË¡¼°¹¦ÄÜ·ÖÎö£ÛJ£Ý. ΢ÉúÎïѧͨ±¨, 2011, 38(11):1679-1687(Zhang J M, Wu H J, Gao X W. Cloning and functional analysis of two type III-secreted effectors in Pseudomonas syringae pv.glycinea£ÛJ£Ý.Microbiology China, 2011, 38(11):1679-1687)

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