|Table of Contents|

Characteristics of Phospholipid Fatty Acids of Soil Microorganism under Different Plant Patterns of Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年03期
Page:
442-448
Research Field:
Publishing date:

Info

Title:
Characteristics of Phospholipid Fatty Acids of Soil Microorganism under Different Plant Patterns of Soybean
Author(s):
YAO Qin12 XU Yan-li1 SONG Jie13 WEI Wei1
1.Key Laboratory of Mollisols Agroecology/Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China;?
2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China;?
3.College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Keywords:
Soybean Rotation Phospholipid fatty acid Soil microorganism
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.03.0442
Abstract:
Phospholipid fatty acid (PLFA) method can accurately express soil microbial groups and biomass. It is a rapid and reproducible analysis method of soil microbial community structure diversity.Based on the long term experimental field at Hailun Agro-ecosystem Experimental Station of Chinese Academy of Sciences, soil microorganism community structure was studied at the flowering stage, seed-filling stage and maturing stage of soybean using the PLFA method under 20 years soybean continuous cropping, rotational cropping, normal rotation and bare land.The results showed that total PLFAs of soil microorganism increased under 20 years soybean continuous cropping.It was significantly higher than other rotation systems, especially at the flowering stage (10 327 μg?g-1).There were no significant differences in total PLFAs at the flowering stage, seed-filling stage and maturing stage of soybean between rotational cropping and normal rotation.Soil fungal PLFAs, bacterial PLFAs and Actinomycetic PLFAs had the similar trend compared with total PLFAs in the whole growth period of soybean.But bacterial PLFAs/fungal PLFAs under 20 years rotational cropping was significantly higher than other rotation systems. It indicated that rotational cropping could induce the increase of bacterial PLFAs and decrease of fungal PLFAs.Principal component analysis (PCA) was adopted to investigate the main soil microorganism PLFAs-PC1 explained 45.5% of the variation, PC2 explained 20.6% of the variation, which helped successfully distinguish rotational cropping and normal rotation from 20 years soybean continuous cropping and bare land in the principal components scores charts. It indicated that 20 years soybean continuous cropping could change soil microorganism community structure. However, soil ecosystem kept relatively stable under 20 years rotational cropping.

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Last Update: 2015-07-16