|Table of Contents|

Optimization on Cotyledonary-node Genetic Transformation System of Soybean Cultivar Kefeng 14(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年03期
Page:
389-393
Research Field:
Publishing date:

Info

Title:
Optimization on Cotyledonary-node Genetic Transformation System of Soybean Cultivar Kefeng 14
Author(s):
WANG WeiYAO Jia-longXIONG He-wenWU Zhi-hui WANG ChengGUO BeiXIE Hao
College of Plant Science and Technology, Beijing University of Agriculture/ Beijing Key Laboratory of New Technology in Agricultural Application, Beijing 102206, China
Keywords:
Kefeng 14 Cotyledonary node Transformation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.03.0389
Abstract:
Kefeng 14 is a big grain type soybean cultivar with a wide adaptability.Based on the genetic transformation system of soybean cotyledonary node mediated by Agrobacterium tumefacines, the influences of the transformation efficiency were analyzed by infection time, 6-BA concentration and the addition of sodium thiosulfate.The results showed that bud rate was up to 72.92% with the optimal infection combination:A tumefacines- concetration at OD600=0.6 with 15 min infection time.The perfect 6-BA and sodium thiosulfate concentration was 1.50 mg?L-1and 1 mmol?L-1?respectively,GUS gene staining technique showed that the above optimized soybean cotyledonary node Agrobacterium-mediated transformation system could improve the transformation efficiency of Kefeng 14.

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Last Update: 2015-07-15