|Table of Contents|

Screening of Homogeneous Transgenic Lines with Na+/H+?Antiporter Gene (AlNHX1) in Soybean and Its Physiological Analysis(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年03期
Page:
378-383
Research Field:
Publishing date:

Info

Title:
Screening of Homogeneous Transgenic Lines with Na+/H+?Antiporter Gene (AlNHX1) in Soybean and Its Physiological Analysis
Author(s):
DONG Li-jun1;  Liu Ling-di2;  CHE Wen-li3;  ZHANG Shu-ling1;  DU Huan1;  GAO Jia-jia1;  LIU Jian-feng1
1.College of Life Science, Hebei University, Baoding 071002，China;?
2. Institute of Cash Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, China;?
3. Crops Institute of Academy of Agricultural and Forestry Sciences of Langfang, Langfang 065000, China
Keywords:
Soybean (Gossypium hirsutum L);  Na+/H+?antiporter gene (AlNHX1);  Transgenic plant;  Salt-tolerance ability
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2015.03.0378
Abstract:
To develop a salt-tolerant soybean cultivar (Glycine max L), a Na+/H+?antiporter gene AlNHX1 from Aeluropus littoralis was successfully expressed in soybean cultivar TF-29. Further, to obtain homogeneous transgenic lines with expression of AlNHX1 gene and to identify the salt-tolerant ability of the transgenic soybean plants, PCR amplification and RT-PCR detection, plant growth performance, K+/Na+?ratio, MDA and H2O2?and the change of antioxidant enzyme activity of transgenic soybean plants were analyzed under hydroponic condition. The results showed that AlNHX1 gene had been integrated into the soybean genome and expressed in transgenic lines．Under 150 mmol?L-1?NaCl salt stress, transgenic lines showed a significant increase in rate of survival, its shoot dry weight and root dry weight reached significant level (P＜0.05 or P＜0.01) compared with wild type plants. The different phenotypes of transgenic soybean and wild type plants were also observed, the transgenics displayed robust performance whereas growth by WT plants was significantly reduced under stress conditions. Also, a higher ratio of K+?to Na+?in the leaves and roots of the transgenic plants was retaining, accompanied with the decline of malondialdehyde (MDA) and hydrogen peroxide (H2O2) and the higher activities of antioxidant enzymes including superoxide dismutase (SOD) and peroxidase (POD) under salt stress.These findings proved that over-expression of exogenous AlNHX1 in soybean have enhanced salt tolerance, and they have significant implications for increasing crop yields on high-salinity soils

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Last Update: 2015-07-15