［1］刘海坤,卫志明.大豆遗传转化研究进展［J］植物生理与生物学报,2005,31(2):126-134（Liu H K, Wei Z M. Recent advance in soybean genetic transformation［J］Journal of Plant Physiology and Molecular Biology,2005,31(2):126-134） ［2］SalmenkallioMarttila M, Aspegren K, Akerman S, et al. Transgenic barley (Hordeum vulgare L) by electroporation of protoplasts［J］ Plant Cell Reports, 1995, 15(3):301-304
［3］Davey M R, Anthony P, Power J B, et al. Plant protoplasts: status and biotechnological perspectives［J］Biotechnology Advances, 2005,23(2): 131-171
［4］Kaeppler H F, Somers D A, Rines H W, et al. Silicon carbide fiber-mediated stable transformation of plant cells［J］TAG Theoretical and Applied Genetics, 1992, 84(5):560-566
［5］Crossway A, Oakes J V, Irvine J M, et al. Integration of foreign DNA following microinjection of tobacco mesophyll protoplasts［J］Molecular and General Genetics MGG, 1986,202(2):179-185
［6］Boynton J E, Gillham N W, Harris E H, et al.Chloroplast transformation in Chlamydomonas with high velocity microprojectiles［J］Science,1988,240(4858):1534-1538
［7］Hinchee M A W, ConnerWard D V, Newell C A, et al. Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer［J］Bio Technology,1993,6:915-922
［8］Okpuzor J ,Omidiji D.Peroxidase-polyphenol oxidase association in Dioscorea esculent［J］Z Naturforsch C, 1998,53(11-12):957-.960
［9］Olhoft P M, Somers D A. L Cysteine increases Agrobacterium-mediated T.DNA delivery into soybean cotyledonary-node cells［J］Plant Cell Report, 2001,20:706-711
［10］Olhoft P M, Flagel L E, Donovan C M, et al. Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method［J］Planta, 2003,5:723-735
［11］陆玲鸿,韩强,李林,等.以草甘膦为筛选标记的大豆转基因体系的建立及抗除草剂转基因大豆的培育［J］中国科学:生命科学,2014(4):406-415 ( Lu L H, Han Q, Li L, et al. Establishment of an efficient transformation protocol for soybean using Glyphosate as selective agent and the development of glyphosate-tolerant transgenic soybean lines［J］Science China, 2014(4):406-415)
［12］Komari T, Hiei Y, Saito Y, et al.Vectors carrying two separate T.DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers［J］The Plant Journal, 1996, 10(1):165-174
［13］BaiX Q, Wang Q Y, Chu C C. Excision of a selective marker in transgenic rice using a novel Cre/loxP system controlled by a floral specific promoter［J］Transgenic Research, 2008,17(6): 1035-1043
［14］YanH, Rommens C M. Transposition-based plant transformation ［J］Plant Physiology, 2007,143(2):570-578
［15］Hooykaas P J, Schilperoort R A. Agrobacterium and plant genetic engineering ［J］ Plant Molecular Biology, 1992,19(1):15-38
［16］Wu C, Sun S, Nimmakayala P, et al.A BAC and BIBAC-based physical map of the soybean genome ［J］Genome Research, 2004,14(2):319-326
［17］Lu H J, Zhou X R,Gong,Z X, et al.Generation of selectable marker-free transgenic rice using double right-border (DRB) binary vectors［J］Functional Plant Biology,2001,28(3):241-248
［18］Matheka J M, Anami S, Gethi J, et al. A new double right border binary vector for producing marker-free transgenic plants［J］BMC Research Notes,2013, 6:448 .
［19］ZhangH B, Wu C C. BAC as tools for genome sequencing ［J］Plant Physiology and Biochemistry, 2001,39(3-4): 195-209
［20］Hamilton C M, Frary A, Lewis C, et al. Stable transfer of intact high molecular weight DNA into plant chromosomes［J］ Proceedings of the National Academy of Sciences of the USA, 1996, 93(18): 9975-9979
［21］Cui Y H, Bi Y M, Brugière N, et al.The S locus glycoprotein and the S receptor kinase are sufficient for self-pollen rejection in Brassica［J］Proceedings of the National Academy of Sciences of the USA, 2000, 97(7):3713-3717.
［22］Frary A, Hamilton C M.Efficiency and stability of high molecular weight DNA transformation: an analysis in tomato［J］Transgenic Research, 2001,10(2): 121-132
［23］He RF, Wang YY, Du B, et al. Development of transformation system of rice based on binary bacterial artificial chromosome (BIBAC) vector ［J］Acta Genetica Sinica,2006,33(3):269-276
［24］Wang W Q， Wu Y R， Li Y，et al. A large insert Thell-ungiella- halophila BIBAC library for genomics and identification of stress tolerance genes［J］Plant Molecular Biology,2010,72(1-2):91-99
［25］Steinrucken H C, Amrhein N. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimate-3-phosphate synthase［J］Biochemistry & Biophysiology,1980,94:1207-1212
［26］Widholm J M，Chinnala A R, Ryu J H, et al. Glyphosate selection of gene amplification in suspension cultures of 3 plant species［J］Physiology Plant,2001,112:540-545
［27］Bayer Crop Science-http://www-bayercropscience-com.
［28］Mathesius C A, Barnett J F J, Cressman R F, et al.Safety assessment-of-a-modified-acetolactate-synthase-protein-(GM-HRA)used-as-a-selectable marker-in-genetically-modified-soybeans［J］ Regulatory Toxicology and Pharmacology,2009,55(3):309-320
［29］van Andreas A. HPPD.Inhibitors-A proven mode of action as a new hope to solve current weed problems ［J］Outlooks on Pest Management, 2009;20(1):27-30
［30］Bohm G M B, Rombaldi C V, Genovese M I, et al. Glyphosate effects on yield,nitrogen fixation,and seed quality in glyphosate-resistant soybean ［J］Crop Science,2014,54:4:1737-1743
［31］岳绍先,刘博林,毛大璋,等.抗阿特拉津转基因大豆植株后代的遗传分析［J］植物学报,1990,32(5):343-349 (Yue S X, Liu B L, Mao D Z, et al.A genetic analysis to the progenies of atrazine-resistance transgenic soybean plant［J］Acta Botanica Sinica, 1990,32(5):343-349)
［32］Liu S C, Zhang G C, Yang L F, et al.Bialaphos-resistant transgenic soybeans produced by the .Agrobacterium-mediated cotyledonary-node method ［J］Journal of Agricultural Science and Technology ,2014, 16: 175-190
［33］胡代花，杨晓伟，冯俊涛，等.大豆食心虫性信息素的研究及应用进展［J］农药学学报，2014（3）：235-244（Hu D H, Yang X W, Feng J T, et al. Advances in the research and application of sex pheromosre Leguminivora glycinivorella Mastunure［J］Chinese Journal of Pesticide Science, 2014(3): 235-244）
［34］Parrot W A, All J N, Adang M J, et al. Recovery and evaluation of soybean plants transgenic for a Bacillus thuringiensis var kurstaki insecticidal gene ［J］In Vitro Cellular & Developmental Biology-Plant, 1994,30:144-149
［35］Stewart C N Jr, Adang M J, All J N, et al.Genetic transformation,recovery,and characterization of fertile soybean transgenic for a synthetic .Bacillus thuringiensis cry1Acgene［J］Plant Physiology,1996,112:121-129
［36］Walker D R, All J N, Mcpherson R M, et al.Field evaluation of soybean engineered with a synthetic cry1Ac transgene for resistance to corn earworm,soybean looper, velvetbean caterpillar (Lepidoptera: Noctuidae),and lesser cornstalk borer (Lepidoptera: Pyralidae) ［J］Journal of Economic Entomology.2000,93(3): 613-622
［37］Mcpherson R M, MacRae T C.Evaluation of transgenic soybean exhibiting high expression of a synthetic .Bacillus thuringiensiscry1Ac transgene for suppressing lepidopteran population densities and crop injury ［J］Journal of Economic Entomology2009,102(4):1640-1648
［38］徐香玲,高晶,刘伟华,等.Ti质粒介导的Bt、kδ内毒素蛋白基因转化大豆的初步研究［J］大豆科学,1997,16(1):7-12(Xu X L, Gao J, Liu W H, et al. Studies on transferring Bt k.Delta endotoxin gene into soybean with Ti-plasmid primaril［J］Soybean Science, 1997,16(1):7-12)
［39］南相日,刘文萍,刘丽艳,等.PEG介导-BT-基因转化大豆原生质体获转基因植株［J］大豆科学,1998,17(4):41-45(Nan X R, Liu W P, Liu L Y, et al.PEG.mediated transformation and regeneration of soybean protoplast with .Bacillus Thuringiensis-Crg-IAc gene ［J］Soybean Science, 1998,17(4):41-45)
［40］任海红,任小俊,王英,等. 外源DNA直接导入技术及在大豆育种中的应用［J］山西农业科学,2013(5):503-505,514(Ren H H, Ren X J, Wang Y, et al.Direct introduction exogenous DNA technique and application in soybean breeding［J］Journal of Shanxi Agricultural Sciences, 2013(5):503-505,514)
［41］杨庆凯,曹越平,崔岩,等.利用花粉管通道技术将抗虫基因导入大豆的研究［J］中国油料作物学报,2002,24(3):17-20(Yang Q K,Cao Y P,Cui Y,et al. Study on introducing Bt gene into soybean by pollen tube path method ［J］Chinese journal of oil crop sciences, 2002,24(3):17-20)
［42］武小霞,刘伟婷,刘琦,等.利用花粉管通道法将抗虫基因(cryV)转入大豆的研究［J］大豆科学,2010,29(4):565-568,574(Wu X X,Liu W T,Liu Q,et al.Introduction of anti pest gene (cry V) to soybean via pollen-tube pathway［J］Soybean Science, 2010,29(4):565-568,574)
［43］杨向东,郭东全,包绍君,等.双价抗虫转基因大豆抗苜蓿夜蛾分析［J农业科学与技术(英文版),2008,9(3):67-69( Yang X D, Guo D Q, Bao S J, et al.Resistance analysis of the binary insect-resistant transgenic soybean to Heliothis viriplaca［J］Agricultural Science & Technology,2008,9(3) : 67-69
［44］Ma X H, Wu T L.Rapid and efficient regeneration in soybean［Glycine max ( L) Merrill］from whole cotyledonary node explants［J］Acta Physiologiae Plantarum,2008,30 ( 2 ): 209-216．
［45］陈秀华,柏锡,潘欣,等.转-cry1Iem-基因大豆的培育及抗虫性检测大豆科学［J］2009,28(6):959-963 (Chen X H, Bai X, Pan X, et al.Cultivation of cry1lem gene transformed soybean and insect resistant assay［J］Soybean Science,2009,28 (6) :959-963)
［46］郑翠明,常汝镇,邱丽娟.大豆花叶病毒病研究进展［J］植物病理学报,2000,30(2):97-105(Zheng C M,Chang R Zh,Qiu L J.Progress on the disease of soybean mosaic virus［J］Acta Phytopathologica sinica, 2000,30(2):97-105)
［47］Schmitthenner A F. Problems and progress in control of Phytop-hthora root rot of soybean［J］Plant Disease, 1985, 69(4): 362-368
［48］徐兆飞,刘亚光.灰斑病菌对大豆叶片总多酚和总黄酮的诱导研究［J］大豆科学,2006,25(3):234-238 (Xu Z F, Liu Y G. Study on lnduction of total podyphenol and flavonoid in soybean leaves by Cercospora sojina-hara［J］Soybean Science, 2006,25(3):234-238)
［49］Abel P P，Nelson R S, De B ,et al. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat proteingene［J］Science,1986,232(4751):738-743
［50］韩阳,王昌凌,赖冰冰.转基因技术在大豆抗病毒育种中的应用研究进展［J］ 江苏农业科学,2010(1):118-120(Han Y, Wang C L, Lai B B.Advances in transgenic technology of antiviral soybean breeding［J］2010(1):118-120)
［51］Di R, Puecell V, Collons G B, et al. Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene［J］Plant Cell Reports,1996,15:746-750
［52］Tougou M, Furutani N, Yamagishi N,et al. Development of resistant transgenic soybeans with inverted repeat-coat protein genes of soybean dwarf virus ［J］Plant Cell Report, 2006;25(11):1213-1218
［53］Tougou M, Yamagishi N, Furutani N,et al.Soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene ［J］Plant Cell Report, 2007,26(11):1967-1975
［54］Cunha W G, Tinoco M L P, Pancoti H L, et al.High resistance to-Sclerotinia sclerotiorum in transgenic soybean plants transformed to express an oxalatedecarboxylase gene ［J Plant Pathology，2010,59(4):654-660
［55］张志忠,吴菁华,吕柳新,等植物几丁质酶及其应用研究进展［J］福建农业大学学报,2005(4):494-499 (Zhang Z Z,Wu J H, Lyu L X, et al.Progress of plant chitnase and its application［J］Journal of Fujian Agricultural University, 2005,04:494-499)
［56］Li H Y, Zhu Y M, Chen Q, et al. Production of trans‐genic soybean plants with two anti-fungal protein genes via -Agrobacterium and particle Bombardment ［J］Biologia Plantarum,2004,48(3):367-374
［57］Steeves R M, Todd T C, Essig J S, et al.Transgenic soybeans expressing siRNAs specific to a major sperm protein gene suppress .Heterodera glycines reproduction［J］Functional Plant Biology, 2006,33:991-999
［58］Li J, Todd T C, Oakley T R, et al. Host-derived suppression of nematode reproductive and fitness genes decreases fecundity of Heterodera glycines Ichinohe［J］Planta,2010,232(3):775-785
［59］Ibrahim H M, Alkharouf N W, Meyer S L, et al.Post-transcriptional gene silencing of root-knot nematode in transformed soybean roots［J］Exp Parasitol, 2011,127(1):90-99
［60］Andrea Maldonado, Reham Youssef, Margaret McDonald, et al.Modification of the expression of two NPR1 suppressors,SNC1 and SNI1,in soybean confers partial resistance to the soybean cyst nematode,Heterodera glycine［J］ Functional Plant Biology, 2014, 41(7) :714-726
［61］刘德璞,李长有.导入外源DNA获得抗SMV大豆品系［J］松辽学刊(自然科学版),1995(3):40-43-(Liu D P, Li C Y.Obtaining anti-SMV soybean lines by introducing foreign DNA-Songliao Journal(Natural Science Edition) 1995(3):40-43)
［62］刘德璞,廖林,袁鹰,等.导入外源DNA获得抗SMV大豆品系［J］大豆科学,1997,16(4):2-7( Liu D P, Liao L, Yuan Y, et al. Gaining of soybean lines resistant to SMV by introducing exogenous DNA［J］Soybean Science, 1997,16(4):2-7)
［63］郭玉双,张艳菊,朱延明,等转几丁质酶和核糖体失活蛋白双价基因大豆的获得与抗病性鉴定［J］作物学报,2006,32(12):1841-1847(Guo Y S, Zhang Y J, Zhu Y M, et al.Obtainment of transgenic soybean plants with chitinase and ribosome inactivating protein genes and their resistance identification［J］Acta Agronomica Sinica,2006,32(12):1841-1847)
［64］张云月,付永平,王丕武,等.转-hrpZpsta-抗病基因大豆的研究［J］西北农林科技大学学报(自然科学版),2011,39(9):86-92(Zhang Y Y,Fu Y P,Wang P W,et al.Study on transforming -hrpZpstagene into soybean［J］Journal of Northwest A&F University(Nat.Sci.Ed), 2011,39(9):86-92)
［65］de Ronde J A, Laurie R N, Caetano T,et al. Comparative study between transgenic and non-transgenic soybean lines proved transgenic lines to be more drought tolerant［J］Euphytica , 2004,138(2): 123-132
［66］Liu M, Li D M, Wang Z K.Transgenic expression of ThIPK2 gene in soybean improves stress tolerance, oleic acid content and seed size［J］Plant Cell Tissue and Organ Culture,2012,111:277-289
［67］Huang X Y, Chao D Y, Gao J P,et al. A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice –via- stomatal aperture control［J］Genes Development,2009,23(15):1805-1817
［68］Schachtman D P, Schroeder J I-Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants［J］Nature ,1994,370:655-658
［69］Yokoi S,Quintero F J, Cubero B,et al.Differential expression and function of Arabidopsis thaliana NHX Na+/H+?antiporters in the salt stress response［J］ Plant Journal,2002,30(5):529-39.
［70］Shi H,Ishitani M, Kim C, et al.The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+?antiporter［J］Proceedings of the National Academyof Sciences of the USA 2000,97:6896-6901
［71］Qi X P, Li M W, Xie M, et al.Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing［J］Nature Communications,2014, 4340
［72］Cao D, Hou W S, Liu W, et al.Overexpression of TaNHX2 enhances salt tolerance of ‘composite’ and whole transgenic soybean plants［J］Plant Cell Tissue and Organ Culture,2011,107:541-552
［73］刘志华,姜振峰,张少良,等.转 –BADH-基因大豆对盐碱土壤氮素转化的影响［J］中国生态农业学报,2014, 22(10): 1200-1206(Liu Z H, Jiang Z F, Zhang S L,et al . Effects of transgenic-BADH-soybean on nitrogen transformation in saline-alkaline soil ［J］Chinese Journal of Eco.Agriculture, 2014, 22(10): 1200-1206)
［74］陈晓军,叶春江,吕慧颖,等.GmHSFA1-基因克隆及其过量表达提高转基因大豆的耐热性［J］遗传,2006,11:1411-1420(Chen X J, Ye C J, Lyu H Y, et al.Cloning ofGmHSFA1 gene and its overexpresion leading to enhancement of heat tolerance in transgenic soybean［J］Hereditas, 2006,11:1411-1420)
［75］曹永强,宋书宏,董丽杰.大豆蛋白质和油分含量遗传研究进展［J］大豆科学,2012,31(2):316-319(Cao Y Q, Song S H, Dong L J. Research progress on heredity of protein and oil content in soybean ［J］Soybean Science, 2012, 31(2):316-319)
［76］Hurburgh C R, Brumm T J, Guinn J M, et al. Protein and oil patterns in US and world soybean markets［J］Journal of the American Oil Chemists Society, 1990, 67(12): 966-973
［77］Lardizabal K, Effertz R, Levering C, et al.Expression of Umbelopsis ramanniana DGAT2A in seed increases oil in soybean［J］ Plant Physiology, 2008, 148(1): 89-96
［78］Kent Brink, ChokFun Chui, Robert F.Molecular characterization, compositional analysis, and germination evaluation of a high-oleic soybean generated by the suppression of FAD2-1 expression［J］Crop Science,2012,54 (5):2160-2174
［79］Zhang L, Yang X D, Zhang Y Y, et al.Changes in oleic acid content of transgenic soybeans by antisense RNA mediated posttranscriptional gene silencing［J］International Journal of Genomics,2014(2014):8
［80］Liu Y F, Li Q T, Lu X,et al. Soybean GmMYB73- promotes lipid accumulation in transgenic plants ［J］BMC Plant Biology,2014, 14:73
［81］El.Shemy H A, Khalafalla M M, Fujita K, et al.Improvement of protein quality in transgenic soybean plants［J］Biologia Plantarum, 2007,51(2):277-284
［82］Kim Y H, Lee Y Y, Kim Y H, et al. Antioxidant activity and inhibition of lipid peroxidation in germinating seeds of transgenic soybean expressing OsHGGT［J］Journal of Agricultural Food Chemisty,2011,59(2):584-591
［83］Kinney A J, Knowlton S.Designer oils: the high oleic acid soybean［M］// Roller S,Harlander S . Genetic modification in the food industry. London: Blackie Academic, 1998:193- 213
［84］Li Z W, Meyer S, Essig J S, et al. High-level expression of maize γzein protein in transgenic soybean (Glycine max)［J］Mol Breed, 2005, 16(1): 11-20
［85］Zhang Y, Schernthaneu J, Labbé N, et al. Improved protein quality in transgenic soybean expression a de novo synthenic protein , MB-16［J］Transgenic Research ,2014, 23(3):455-467
［86］Sato S, Xing A Q, Ye X G, et al.Production of γ-linolenic acid and stearidonic acid in seeds of marker-free transgenic soybean［J］Crop Science, 2004, 44(2): 646-652
［87］Eckert H, LaVallee B, Schweiger B J, et al.Co-expression of the borage Δ6 desaturase and the Arabidopsis Δ15 desaturase results in high accumulation of stearidonic acid in the seeds of transgenic soybean［J］Planta, 2006, 224(5): 1050-1057
［88］Chiera J M, Finer J J, Grabau E A.Ectopic expression of a soybeanphytase in developing seeds of Glycine max to improve phosphorus availability［J］Plant Molecular Biology, 2004, 56(6): 895-904
［89］雷勃钧,李希臣,卢翠华,等.外源野生大豆DNA导入栽培大豆及RAPD分子验证［J］中国科学(B辑 化学 生命科学 地学),1994(6):596-601(Lei B J, Li X C ,Lu C H, et al. Wild soybean exogenous DNA transferring into planting soybeans and RAPD molecular validation［J］Science in China(Series B),1994(6):596-601)
［90］Wang Z K, Huang W J, Chang J M, et al. Overexpression of SiDGAT1, a gene encoding acyl CoA:diacylglycerol acyltransferase from -Sesamum indicum. L. increases oil content in transgenic Arabidopsis and soybean［J］Plant Cell Tissue and Organ Culture,2014, DOI 10-1007/s 11240-014-0543-z
［91］Ding S H, Huang L Y, Wang Y D, et al. High-level expression of basicfibroblast growth factor in transgenic soybean seeds and characterization of its biological activity［J］Biotechnol Lett, 2006,28(12):869-875
［92］Judy L O, Kenneth L B, Kenneth J P.Stability of a soybean seed-derived vaccine antigen following long-term storage, processing and transport in the absence of a cold chain ［J］Journal of the Science of Food and Agriculture,2009,89(13):2191-2199
［93］Cunha N B, Araujo A C, Leite A, et al. Correct targeting of proinsulin in protein storage vacuoles of transgenic soybean seeds［J］ Genetic Molecular Research,2010,9(2):1163-1170
［94］Cunha N B, Murad A M, Cipriano T M, et al.Expression of functional recombinant human growth hormone in transgenic soybean seeds ［J］ Transgenic Research, 2011,20:811-826
［95］Nobuyuki Maruyama, Keigo Fujiwara, Kazunori Yokoyama,et al. Stable accumulation of seed storage proteins containing vaccine peptides in transgenic soybean seeds ［J］Journal of Bioscience and Bioengineering, 2014, 118(4):441-447