|Table of Contents|

Removal of Trypsin Inhibitor from Soy Molasses Supernatant(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年02期
Page:
298-301
Research Field:
Publishing date:

Info

Title:
Removal of Trypsin Inhibitor from Soy Molasses Supernatant
Author(s):
SHI Yun;  KONG Xiang-zhen;  HUA Yu-fei
School of Food Science and Technology, Jiangnan University/State Key Laboratory of Food Science and Technology,Wuxi 214122, China
Keywords:
Soy molasses;  Tripsin inhibitor;  Tricine-SDS-PAGE;  Ultrafiltration;  Soy oligosaccharides
PACS:
TS201.2
DOI:
10.11861/j.issn.1000-9841.2015.02.0298
Abstract:
Trypsin inhibitor is one kind of antinutrition factor widely spread in soybean and soybean products, which mainly consist of Kuntiz type and Bowman-Birk type. In order to get high-quality products, soybean trypsin inhibitor is better to be inactivated or removed.Through analyzing the activity and identifying the type of trypsin inhibitor in soy molasses supernatant, it was removed using ultrafiltration.The results showed that the trypsin inhibitor activity for soy molasses supernatant with about 10% solids content was approximately 5.05×105?TIA?g-1?protein, which was nearly 5 times of fresh soy milk whose trypsin inhibitor activity was about 1.026×105?TIA?g-1?protein. Protein with high molecular weight in soy molasses supernatant was concentrated by ultrafiltration using a membrane with a molecular weight cut-off of 3 kDa. The retentates showed one, unclear band with a molecular weight of about 10 kDa on the chromatogram of reduced Tricine.SDS-PAGE-After reduced by 2% mercaptoethanol and blocked thiol by NEM, the retentates of soy molasses supernatant showed two, clear bands with molecular weights of about 7.8 kDa Combined the facts, it was reasonable to consider that there was mainly Bowman-Birk trypsin inhibitor in soy molasses supernatant.Finally, ultrafiltrtion membrane with a molecular weight cut-off of 10 kDa was chosen to retain the trypsin inhibitor in soy molasses supernatant, and therefore permeates without inhibitor activity could be obtained.

References:

［1］Hosny M, Rosazza P N. Novel isoflavone, cinnamic acid, and triterpenoid glycosides in soybean molasses ［J］Journal of Natural Products, 1999, 62: 853-858

［2］Nash A M, Eldridge A C, Wolf W J.Fractionation and characterization of alcohol extractables associated with soybean proteins: Nonprotein components ［J］Journal of Agriculture and Food Chemistry, 1967, 15: 102-108
［3］Lan Y, Williams B A, Verstegen M W A, et al.Soy oligosaccharides in vitro fementation characteristics and its effect on caecal microorganisms of young broiler chickens ［J］Animal Feed Science and Technology, 2007, 133: 286-297
［4］袁其朋，张恭孝，姜焱.树脂及活性碳吸附技术回收大豆乳清中的异黄酮和低聚糖［J］大豆科学, 2003, 22（1）: 6-9 (Yuan Q P, Zhang G X, Jiang Y. Recovery of soya isoflavones and oligosaccharide from soybean whey wastewater by adsorption and desorption of resin and active carbon ［J］Soybean Science, 2003, 22（1）: 6-9)
［5］高文宏，石彦国，高大维，等. 超滤法提取大豆低聚糖前处理的研究［J］中国粮油学报, 2000, 15（5）: 49-51 (Gao W H, Shi Y G, Gao D W, et al. Pretreatment of extracting soybean oligosaccharides by ultrafiltration ［J］Jouranl of the Chinese Cereals and Oils Association, 2000, 15（5）: 49-51)
［6］Shi Y, Kong X Z, Hua Y F, et al. Adsorption of soy isoflavones by activated carbon: Kinetics, thermodynamics and influence of soy oligosaccharides ［J］Chemical Engineering Journal, 2013, 215-216: 113-121
［7］谷春梅，韩玲玲，曲洪生，等.大豆胰蛋白酶抑制因子的研究进展［J］大豆科学, 2012, 31(1): 149-151 (Gu C M, Han L L, Qu H S, et al.Extraction and purification of soybean trypsin inhibitor ［J］Soybean Science, 2012, 31(1): 149-151)
［8］金蓓，田少君.大豆胰蛋白酶抑制剂研究概况［J］粮食与油脂, 2005(6): 3-6 (Jin B, Tian S J.Research survey in soybean trypsin inhibitor ［J］Journal of Cereals & Oils, 2005(6): 3-6)
［9］方伟辉，华欲飞，陶冠军，等.大豆粕酒精可溶物的成分分离与鉴定［J］中国油脂, 2004, 29(1): 57-61 (Fang W H, Hua Y F, Tao G J, et al.Fractionation and identification in alcohol soluble of soybean meal ［J］China Oils and Fats, 2004(1), 29: 57-61)
［10］崔希庆.大豆糖蜜中功能性低聚糖的纯化分离［D］哈尔滨：东北农业大学， 2010 (Cui X Q. Purification and separation of functional oligosaccharides from soybean molasses ［D］Harbin: Northeast Agricultural University, 2010
［11］张水华.食品分析［M］北京: 中国轻工业出版社, 2004: 156-159 (Zhang S H. Food analysis ［M］Beijing: China Light Industry Press, 2004: 156-159)
［12］刘立超，黄洪林，赖正权，等.苯酚硫酸法测定栀子水溶性多糖含量［J］安徽医药, 2005(9): 831-832 (Liu L C, Huang H L, Lai Z Q, et al. Determination of water soluble polysaccharide in fructus gardenia ［J］Anhui Medical and Pharmaceutical Journal, 2005(9): 831-832)
［13］Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 ［J］Nature, 1970, 227: 680-685
［14］Hermann Sch.gger Tricene-SDS-PAGE ［J］Nature Protocols, 2006, 1: 16-22
［15］黄惠华，梁汉华，郭乾初.两种大豆胰蛋白酶抑制剂的抑制活性及二级结构分析比较［J］食品科学, 2005, 26(3): 46-49 (Huang H H, Liang H H, Guo Q C. Inhibitory activities and secondary structures of two types of soybean trypsin inhibitors ［J］Chinese Food Science, 2005, 26(3): 46-49)
［16］Li X F, Dong, D Hua Y F, et al.Soybean whey protein/chitosan complex behavior and selective recovery of kunitz trypsin inhibitor ［J］Journal of Agriculture and Food Chemistry, 2014, 62: 7279-7286
［17］黄惠华，周春晖.热处理对鲜豆奶中胰蛋白酶抑制子活性的影响［J］食品科技, 2001(3): 7-9 (Huang H H, Zhou C H.Effect of thermal treatment on trypsin inhibitor activity of soybean ［J］Food Science and Technology, 2001(3): 7-9)
［18］万娟，陈中，杨晓泉，等. 不同还原剂对大豆胰蛋白酶抑制剂的钝化研究［J］食品工业科技, 2007, 28(3): 82-84 (Wan J, Chen Z, Yang X Q, et al.Study on the inactivation of soybean trypsin inhibitor by various reducing agents ［J］Science and Technology of Food Industry, 2007, 28(3): 82-84)

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Last Update: 2015-06-08