|Table of Contents|

Aanalysis of Drought Tolerance in Transgenic Soybean with GmHAP3-17(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年02期
Page:
249-254
Research Field:
Publishing date:

Info

Title:
Aanalysis of Drought Tolerance in Transgenic Soybean with GmHAP3-17
Author(s):
WANG Ding-hui12 LI Yong-guang1 LI Zhen1 LI Wen-bin1
1College of Life Sciences, Liaoning Normal University, Dalian 116029, China;?
2 Key Laboratory of Soybean Biology in Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry, Northeast Agricultural University, Harbin 150030,China
Keywords:
GmHAP3-17 Soybean Drought tolerance Gene function analysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0249
Abstract:
The present research work was mainly aim at analysing the morphological and physiological response under drought stress in transgenic soybean with GmHAP3-17. The results showed that under water-limited conditions of greenhouse, comparing with wild-type, the H16, H18 and H26 lines of transgenic soybean appeared better growth status, wilting later and milder, longer taproot, more lateral roots, lower degree of leaf damage, lower concentration of MDA and higher content of leaf relative water. Future experiment proved that under natural drought conditions of dry regions, comparing with wild-type, the root system of transgenic soybeans developed well, grew rapidly, and showed longer taproot, more lateral roots. The plants of transgenic soybean were higher in plant height, more in the number of branch and main stem node, more in pods and kernels, larger in seed size and hundred-grain weight. These consequences indicated that the GmHAP3-17 gene functions were positive regulation after drought stress treatment and thus, it could be useful in molecular breeding as effective genetic sources of transgenic crops.

References:

[1]Bruce W B, Edmeades G O, Barker T C.Molecular and physiological approaches to maize improvement for drought tolerance[J]Journal of Experimental Botany, 2002, 53:13-25

[2]刘强,赵南明,Yamaguchi Shinozaki K, 等.DREB转录因子在提高植物抗逆性中的作用[J]科学通报,2000, 45(1): 11-16(Li Q, Zhao N M, Yamaguchi-Shinozaki K, et al. Function of DREB transcription factors in improving the plant resistance[J], Science Bulletin, 2000, 45(1): 11-16
[3]YamaguchiShinozaki K, Kasuga M, Liu Q. Biological mechanisms of drought stress response[J]JIRCAS Working Report, 2002, 9: 1-8
[4]Donald E N, Peter P R, Tom R A. Plant nuclear factor Y (NF.Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres[J]Proceeding of the National Academy of Science of the United States of America, 2007, 104(42):16450-16455
[5]Mantovani R.The molecular biology of the CCAAT.binding factor NF.Y[J]Gene, 1999, 239:15-27
[6]Siefers N, Dang K K, Kumimoto R W.Tissue-specific expression patterns of Arabidopsis NF Y transcription factors suggest potential for extensive combinatorial complexity[J] Plant Physiology- 2009, 149(2):625-641
[7]Ni Z, Hu Z, Jiang Q, et al. GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress[J] Plant Molecular Biology, 2013, 82(1-2):113-29
[8]Pennisi E. Plant genetics Getting to the root of drought responses[J]Science, 2008, 11:171-173
[9]Huang C Y, Kuchel H, Edwards J. A DNA.based method for studying root responses to drought in field-grown wheat genotypes[J]Scientific Reports, 2013, 3:3194
[10]LeRudulier D, Strom A R, Dandekar A M.Molecular biology of osmoregulation[J] Science, 1989, 224:1064-1068
[11]Ni Z, Hu Z, Jiang Q, et al.GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress[J]Plant Molecular Biology, 2013,82(1-2):113-129
[12]Wang C, Zhang D W, Wang Y C.A glycine-rich RNA-binding protein can mediate physiological responses in transgenic plants under salt stress[J]Molecular Biology Reports, 2012, 39(2):1047-1053
[13]Gadjev I, Vanderauwera S, Gechev T S, et al.Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis[J]Plant Physiology, 2006, 141(2):436-445

Memo

Memo:
-
Last Update: 2015-06-07