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Construction of the Folate Biosynthetic Pathway folE Gene and Transformation in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年02期
Page:
224-227
Research Field:
Publishing date:

Info

Title:
Construction of the Folate Biosynthetic Pathway folE Gene and Transformation in Soybean
Author(s):
ZHANG Yuan-yu WANG Yu-min YU Zhi-jing LI Hai-yun ZHANG Ling
AgroBiotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China
Keywords:
Soybean Folate folE Transgenic
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0224
Abstract:
The folE is a key gene of folic acid pathway, which is derived from bacteria.The function of this gene in the improved folate content has been demonstrated in many plants,such as tomato In this study, we expressed the -folE-by employing the soybean cotyledonary nodes method.We co-cultivated about 200 explants, and got 34 transgenic plants. Stock of Basta (135 mg?L-1) was painted on half the upper surface of tested soybean leaves, and got 14 transgenic plants. PCR analysis showed that 12 of 14 transgenic plants were positive Southern blot analysis of PCRpositive plants indicated the integration of the-folE- gene into the soybean genome, and we also got two transgenic lines with only one single copy.

References:

[1]HansonA D,Gregory J F.Synthesis and turnover of folates in plants[J]Current Opinion in Plant Biology, 2002, 5(3): 244-253

[2]Storozhenko S, De Brouwer V, Volckaert M, et al. Folate fortification of rice by metabolic engineering[J]Nature Biotechnology,2007, 25(11): 1277-1286
[3]Geisel J.Folic acid and neural tube defects in pregnancy[R]The Journal of Perinatal and Neonatal Nursing, 2003, 17(4): 268-271
[4]Choi S W, Friso S. Interactions between folate and aging for carcinogenesis[J] Clinical Chemistry and Laboratory Medicine, 2005, 43(10): 1151-1157
[5]McIntosh S R, Brushett D, Henry R J.GTPcyclohydrolase I expression and folate accumulation in the developing wheat seed[J]Journal of Cereal Science, 2008, 48: 503-509
[6]Basset G, Quinlivan E P, Ziemak M J, et al. Folate synthesis in plants: The first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I[J] Proceeding of the National Academy of Sciences of the United States of America, 2002, 99(19): 12489-12594
[7]Goyer A, Illarionova V, Roje S, et al. Folate biosynthesis in higher plants-cDNA cloning, heterologous expression, and characterization of dihydroneopterin aldolases[J] Plant Physiology, 2004, 135(1): 103-111
[8]Goyer A, Navarre D A.Determination of folate concentrations in diverse potato germplasm using a trienzyme extration and a microbiological assay[J]Journal Agricultural Food Chemistry, 2007, 55(9): 3523-3528
[9]Sybesma W, Starrenburg M, Kleerebezem M, et al. Increased production of folate by metabolic engineering of Lactococcus lactis[J]Applied and Environment Microbiology, 2003, 69(6): 3069-3076
[10]Díaz de la Garza R I, Gregory J F, Hanson A D.Folate biofortification of tomato fruit [J]Proceeding of the National Academy of Sciences of the United States of America, 2007, 104(10): 4218-4238
[11]Olhoft P M, Flangel L E, Donovan C M, et al. Efficient soybean transformation using hygromycin B selectionin the cotyledonary-node method[J] Planta, 2003, 216(5): 723-735
[12]许丽惠,谢曲丽,林丽花.叶酸的研究进展[J]福建畜牧兽医, 2013,35(2):34-36(Xu L H, Xie Q L, Lin L H. Advancement of researches on folate[J]Fujian Journal of Animal Husbandry and Veterinary Medicine, 2013, 35(2): 34-36
[13]常娜宁,姜凌,蒲训,等.作物叶酸检测方法的研究进展[J]中国农业科技导报, 2010,12(2):44-49(Chang N N, Jiang L, Pu X, et al.Research progress oil methodology for folate detection in crops[J]Journal of Agricultural Science and Technology, 2010, 12(2): 44-49)
[14]Basset G J, Ravanel S, Quinlivan E P, et al. Folate synthesis in plants: The last step of the p-aminobenzoate branch is catalyzed by a plastidial aminodeoxychorismate lyase[J] Plant Journal, 2004, 40(4): 453-461
[15]Bekaert S, Storozhenko S, Mehrshahi P, et al. Folate biofortification in food plants[J] Trendsin Plant Science, 2008, 13(1): 28-35
[16]Bello A R, Nare B, Freedman D, et al. PTR1: A reductase mediating salvage of oxidized pteridines and methotrexate resistance in the protozoan parasite Leishmania major[J] Proceeding of the National Academy of Sciences of the United States of America, 1994, 91(24): 11442-11446
[17]Bermingham A, Derrick J P.The folic acid biosynthesis pathway in bacteria: evaluation of potential for antibacterial drug discovery [J]Bioessays, 2002, 24(7): 637-648

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Last Update: 2015-06-07